| Autor: |
Wada H; Associated Department with Mie Graduate School of Medicine, Mie Prefectural General Medical Center, Yokkaichi 510-0885, Japan., Shiraki K; Associated Department with Mie Graduate School of Medicine, Mie Prefectural General Medical Center, Yokkaichi 510-0885, Japan.; Department of General Medicine, Mie Prefectural General Medical Center, Yokkaichi 510-0885, Japan., Matsumoto T; Department of Transfusion Medicine and Cell Therapy, Mie University Hospital, Tsu 514-8507, Japan., Shimpo H; Mie Prefectural General Medical Center, Yokkaichi 510-0885, Japan., Sakano Y; Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan., Nishii H; Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan., Tamaki S; Department of Hematology, Japanese Red Cross Ise Hospital, Ise 516-8512, Japan., Suzuki K; The Advanced Emergency and Critical Care Center, Mie University Hospital, Tsu 514-8507, Japan., Tawara I; Department of Hematology and Oncology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan., Yamashita Y; Department of Hematology and Oncology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan., Shimaoka M; Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan. |
| Abstrakt: |
Background/Objectives : FVIII reagent activity varies across different assays, as well as activated partial thromboplastin time (APTT) reagents. The hemostatic ability of various FVIII reagents was examined via clot waveform analysis (CWA). Methods : APTT was measured using 12 APTT reagents, a small amount of tissue factor-induced FIX activation (sTF/FIXa) and a small amount of thrombin time (sTT) in order to examine 10 FVIII reagents and reference plasma (RP) using CWA. FVIII activity was measured using CWA-APTT, a chromogenic assay, or CWA-sTT. Results : Although the peak time (PT) and peak height (PH) of the CWA-APTT were markedly different in different FVIII reagents using several APTT reagents, the PTs of CWA-APTT were generally normal or shortened and the PHs of CWA-APTT were generally lower than those of RP. The FVIII activity varied, as evaluated using APTT, and was higher when using the CWA-sTT method than the APTT or chromogenic methods. CWA-sTT showed an elevated second peak of first DPH in all FVIII reagents, and both CWA-sTF/FIXa and CWA-sTT were enhanced using APTT reagents. Conclusions: Our evaluation of the hemostatic ability of FVIII reagents varied among APTT reagents. CWA-sTT can be used to further evaluate the hemostatic ability of an FVIII concentrate based on thrombin burst. |
