An Analytical Protocol for Detecting Antibody Titer Levels in Serum/Saliva by Indirect Enzyme-Linked Immunosorbent Assay (ELISA).

Autor:	Wang J; The University of Queensland, School of Chemistry and Molecular Biosciences, St Lucia, QLD, Australia., Bashiri S; The University of Queensland, School of Chemistry and Molecular Biosciences, St Lucia, QLD, Australia., Toth I; The University of Queensland, School of Chemistry and Molecular Biosciences, St Lucia, QLD, Australia. i.toth@uq.edu.au.; The University of Queensland, Institute of Molecular Biosciences, St Lucia, QLD, Australia. i.toth@uq.edu.au.; The University of Queensland, School of Pharmacy, Woolloongabba, QLD, Australia. i.toth@uq.edu.au., Skwarczynski M; The University of Queensland, School of Chemistry and Molecular Biosciences, St Lucia, QLD, Australia. m.skwarczynski@uq.edu.au.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2821, pp. 205-216.
DOI:	10.1007/978-1-0716-3914-6_16
Abstrakt:	Enzyme-linked immunosorbent assay (ELISA) detects qualitatively and quantitatively the presence of antibodies or antigens in a sample. Due to its simplicity, high sensitivity, and user-friendliness, the test is widely used in laboratory research, clinical diagnoses, and food testing. This chapter describes the indirect semiquantitative ELISA protocol used to monitor antibody levels in animals and analyze the titer levels of specific antibodies against a target antigen in serum and saliva. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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