Pipette-tip solid-phase extraction coupled with matrix-assisted laser desorption/ionization mass spectrometry enables rapid and high-throughput analysis of antidepressants in rat serum.

Autor: Sun Z; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China., Wang F; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China., Li W; Henan Key Laboratory of Nanomedicine for Targeting Diagnosis and Treatment, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, China., Ren R; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China., Zhou P; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China., Jia Q; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China., Zhao L; Shenzhen Bao'an District Center for Disease Control and Prevention, Shenzhen, 518101, China., Chen D; Henan Key Laboratory of Nanomedicine for Targeting Diagnosis and Treatment, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, China. dichen@zzu.edu.cn., Zuo L; Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. zuolihua2013@126.com.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2024 Sep; Vol. 416 (22), pp. 5013-5023. Date of Electronic Publication: 2024 Jul 13.
DOI: 10.1007/s00216-024-05439-x
Abstrakt: Therapeutic drug monitoring is essential for ensuring the efficacy and safety of medications. This study introduces a streamlined approach that combines pipette-tip solid-phase extraction (PT-SPE) with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), facilitating rapid and high-throughput monitoring of drug concentrations. As a demonstration, this method was applied to the extraction and quantification of antidepressants in serum. Utilizing Zip-Tip C18, the method enabled the extraction of antidepressants from complex biological matrices in less than 2 min, with the subsequent MALDI-MS analysis yielding results in just 1 min. Optimal extraction recoveries were achieved using a sampling solution at pH 9.0 and a 10 μL ethanol desorption solution containing 0.1% phosphoric acid. For MALDI analysis, 2,5-dihydroxybenzoic acid was identified as the most effective matrix for producing the highest signal intensity. The quantification strategy exhibited robust linearities (R 2  ≥ 0.997) and satisfactory limits of quantification, ranging from 0.05 to 0.5 μg/mL for a suite of antidepressants. The application for monitoring dynamic concentration changes of antidepressants in rat serum emphasized the method's efficacy. This strategy offers the advantages of high throughput, minimal sample usage, environmental sustainability, and simplicity, providing ideas and a reference basis for the subsequent development of methods for therapeutic drug monitoring.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
Databáze: MEDLINE
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