Arginine vasopressin activates serotonergic neurons in the dorsal raphe nucleus during neonatal development in vitro and in vivo.

Autor: Orav E; HiLIFE Neuroscience Center, University of Helsinki, Helsinki, Finland. Electronic address: ester.orav@gmail.com., Kokinovic B; HiLIFE Neuroscience Center, University of Helsinki, Helsinki, Finland. Electronic address: bojana.kokinovic@helsinki.fi., Teppola H; HiLIFE Neuroscience Center, University of Helsinki, Helsinki, Finland. Electronic address: heidi.teppola@oist.jp., Siimon M; HiLIFE Neuroscience Center, University of Helsinki, Helsinki, Finland. Electronic address: marisiimon01@gmail.com., Lauri SE; HiLIFE Neuroscience Center, University of Helsinki, Helsinki, Finland. Electronic address: sari.lauri@helsinki.fi., Hartung H; HiLIFE Neuroscience Center, University of Helsinki, Helsinki, Finland. Electronic address: henrike.hartung@helsinki.fi.
Jazyk: angličtina
Zdroj: Neuropharmacology [Neuropharmacology] 2024 Nov 01; Vol. 258, pp. 110068. Date of Electronic Publication: 2024 Jul 10.
DOI: 10.1016/j.neuropharm.2024.110068
Abstrakt: Birth stress is a risk factor for psychiatric disorders and associated with exaggerated release of the stress hormone arginine vasopressin (AVP) into circulation and in the brain. In perinatal hippocampus, AVP activates GABAergic interneurons which leads to suppression of spontaneous network events and suggests a protective function of AVP on cortical networks during birth. However, the role of AVP in developing subcortical networks is not known. Here we tested the effect of AVP on the dorsal raphe nucleus (DRN) 5-hydroxytryptamine (5-HT, serotonin) system in male and female neonatal rats, since early 5-HT homeostasis is critical for the development of cortical brain regions and emotional behaviors. We show that AVP is strongly excitatory in neonatal DRN: it increases excitatory synaptic inputs of 5-HT neurons via V 1A receptors in vitro and promotes their action potential firing through a combination of its effect on glutamatergic synaptic transmission and a direct effect on the excitability of these neurons. Furthermore, we identified two major firing patterns of neonatal 5-HT neurons in vivo, tonic regular firing and low frequency oscillations of regular spike trains and confirmed that these neurons are also activated by AVP in vivo. Finally, we show that the sparse vasopressinergic innervation in neonatal DRN originates exclusively from cell groups in medial amygdala and bed nucleus of stria terminalis. Hyperactivation of the neonatal 5-HT system by AVP during birth stress may impact its own functional development and affect the maturation of cortical target regions, which may increase the risk for psychiatric conditions later on.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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