Molecular and geochemical basis of microbially induced carbonate precipitation for treating acid mine drainage: The case of a novel Sporosarcina genomospecies from mine tailings.

Autor: Cuaxinque-Flores G; Doctorado en Recursos Naturales y Ecologia, Facultad de Ecología Marina, Universidad Autónoma de Guerrero, Gran vía tropical 20, Fraccionamiento Las playas, Acapulco de Juárez, Guerrero, Mexico., Talavera-Mendoza O; Doctorado en Recursos Naturales y Ecologia, Facultad de Ecología Marina, Universidad Autónoma de Guerrero, Gran vía tropical 20, Fraccionamiento Las playas, Acapulco de Juárez, Guerrero, Mexico; Escuela Superior de Ciencias de la Tierra, Universidad Autónoma de Guerrero, Ex-hacienda, San Juan Bautista s/n, CP 40323 Taxco el Viejo, Guerrero, Mexico. Electronic address: otalaver.uagro@gmail.com., Aguirre-Noyola JL; Centro Nacional de Recursos Genéticos, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Tepatitlán de Morelos 47600, Jalisco, Mexico., Hernández-Flores G; CONAHCyT-Escuela Superior de Ciencias de la Tierra, Universidad Autónoma de Guerrero, Ex Hacienda San Juan Bautista s/n, Taxco de Alarcón 40323, Mexico., Martínez-Miranda V; Instituto Interamericano de Tecnología y Ciencias del Agua (IITCA), Universidad Autónoma del Estado de México, Unidad San Cayetano, Km. 14.5, Carretera, Toluca-Atlacomulco, C.P. 50200 Toluca, Estado de México, Mexico., Rosas-Guerrero V; Escuela Superior en Desarrollo Sustentable, Universidad Autónoma de Guerrero, Tecpan de Galeana 40900, Mexico., Martínez-Romero E; Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, UNAM, Av. Universidad s/n, Chamilpa, 62210 Cuernavaca, Morelos, Mexico. Electronic address: emartine@ccg.unam.mx.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2024 Sep 05; Vol. 476, pp. 135005. Date of Electronic Publication: 2024 Jun 28.
DOI: 10.1016/j.jhazmat.2024.135005
Abstrakt: Microbially induced carbonate precipitation (MICP) immobilizes toxic metals and reduces their bioavailability in aqueous systems. However, its application in the treatment of acid mine drainage (AMD) is poorly understood. In this study, the genomes of Sporosarcina sp. UB5 and UB10 were sequenced. Urease, carbonic anhydrases, and metal resistance genes were identified and enzymatic assays were performed for their validation. The geochemical mechanism of precipitation in AMD was elucidated through geo-mineralogical analysis. Sporosarcina sp. UB5 was shown to be a new genomospecies, with an average nucleotide identity < 95 % (ANI) and DNA-DNA hybridization < 70 % (DDH) whereas UB10 is close to S. pasteurii. UB5 contained two urease operons, whereas only one was identified in UB10. The ureolytic activities of UB5 and UB10 were 122.67 ± 15.74 and 131.70 ± 14.35 mM NH 4 + min -1 , respectively. Both strains feature several carbonic anhydrases of the α, β, or γ families, which catalyzed the precipitation of CaCO 3 . Only Sporosarcina sp. UB5 was able to immobilize metals and neutralize AMD. Geo-mineralogical analyses revealed that UB5 directly immobilized Fe (1-23 %), Mn (0.65-1.33 %) and Zn (0.8-3 %) in AMD via MICP and indirectly through adsorption to calcite and binding to bacterial cell walls. The MICP-treated AMD exhibited high removal rates (>67 %) for Ag, Al, As, Ca, Cd, Co, Cu, Fe, Mn, Pb, and Zn, and a removal rate of 15 % for Mg. This study provides new insights into the MICP process and its applications to AMD treatment using autochthonous strains.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: