A down-regulated cytochrome P450 in Neoseiulus barkeri Hughes (Acari: Phytoseiidae) can dechlorinate and hydroxylate chlorpyrifos without producing chlorpyrifos-oxon.

Autor: Yu SJ; Citrus Research Institute, Southwest University, National Citrus Engineering Research Center, Chongqing 400712, China., Wang L; Chongqing Chemical Industry Vocational College, Chongqing 401228, China., Ding LL; Citrus Research Institute, Southwest University, National Citrus Engineering Research Center, Chongqing 400712, China., Pan Q; Citrus Research Institute, Southwest University, National Citrus Engineering Research Center, Chongqing 400712, China., Li SC; Citrus Research Institute, Southwest University, National Citrus Engineering Research Center, Chongqing 400712, China., Liu L; Citrus Research Institute, Southwest University, National Citrus Engineering Research Center, Chongqing 400712, China., Cong L; Citrus Research Institute, Southwest University, National Citrus Engineering Research Center, Chongqing 400712, China., Ran C; Citrus Research Institute, Southwest University, National Citrus Engineering Research Center, Chongqing 400712, China. Electronic address: ranchun@cric.cn.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2024 Sep 05; Vol. 476, pp. 135163. Date of Electronic Publication: 2024 Jul 09.
DOI: 10.1016/j.jhazmat.2024.135163
Abstrakt: Selection of chemical-resistant predatory mites is a good alternative to balance the contradiction between chemical control and biological control. Previously, a resistant strain of Neoseiulus barkeri for chlorpyrifos was obtained. In the current study, two up-regulated (NbCYP3A6, NbCYP3A16) and one down-regulated (NbCYP3A24) P450s were screened through differential expression analysis and other detoxification-related genes such as CCEs, GST, etc. were not found. 3D modelling and molecular docking indicated that the chlorine at position 5 on the pyridine ring of chlorpyrifos, as well as a methyl group, were closest to the heme iron of the enzymes (less than 5 Å). Three active recombinant P450 proteins were heterologously expressed and metabolized with chlorpyrifos in vitro. HPLC assay showed that only NbCYP3A24 could metabolize chlorpyrifos, with a metabolism rate of 21.60 %. Analysis of the m/z of metabolites by LC-MS/MS showed that chlorine at the 5 C position of chlorpyrifos was stripped and hydroxylated, whereas chlorpyrifos-oxon, a common product of oxidation by P450, was not found. Knockdown of the NbCYP3A24 gene in the susceptiblestrain did reduce the susceptibility of N. barkeri to chlorpyrifos, suggesting that the biological activity of the metabolite may be similar to chlorpyrifos-oxon, thus enhancing the inhibitory effect on the target.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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