Post-cooling sperm processing can rescue sperm quality of cooled-stored stallion semen.

Autor: Segabinazzi LGTM; São Paulo State University, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo, Brazil; Center 2, Ross University School of Veterinary Medicine, PO Box 334, Basseterre, St. Kitts, USA. Electronic address: LSegabinazzi@rossvet.edu.kn., Dell'Aqua CPF; São Paulo State University, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo, Brazil., Cavalero T; São Paulo State University, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo, Brazil., Frasson M; São Paulo State University, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo, Brazil., Lisboa FP; São Paulo State University, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo, Brazil; El Rincon Centro de Reproduccion Equina, Marco Paz, Buenos Aires, Argentina., Papa FO; São Paulo State University, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo, Brazil., Alvarenga MA; São Paulo State University, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2024 Oct 01; Vol. 227, pp. 21-30. Date of Electronic Publication: 2024 Jul 08.
DOI: 10.1016/j.theriogenology.2024.07.004
Abstrakt: Poor sperm quality in cooled-shipped semen has been related to subpar fertility in horses. Therefore, this study aimed to evaluate the ability of post-cooling sperm processing to improve sperm parameters of cooled-stored stallion semen for artificial insemination. For all experiments, ejaculates were collected, processed, and diluted in skimmed milk-based (SM) medium and stored at 5 °C/24h. In all experiments an aliquot of unprocessed cooled semen was used as a control. In the first experiment (Exp 1.), cooled-stored semen from 16 stallions (n = 32) was processed by SpermFilter or centrifugation (600×g/10min) and resuspended in an egg yolk-based freezing medium containing permeating cryoprotectants (EY-C) for cryopreservation. Sperm recovery and motility parameters were immediately assessed after sperm resuspension in both groups and compared with unprocessed (Unp) samples. In Exp 2., cooled semen samples from six stallions (n = 18) were processed using SpermFilter and resuspended in SM or EY-C. Motility parameters and plasma membrane integrity were assessed in all groups (Unp, SM, and EY-C). In Exp 3, cooled semen from four stallions (n = 20) was processed by SpermFilter, resuspended in SM, EY-C, or egg yolk-based medium without cryoprotectants (EY-nC); and submitted to a thermoresistance test (37 °C/3h). Motility parameters, plasma membrane integrity and stability, mitochondrial membrane potential, mitochondrial superoxide generation, and DNA fragmentation index were evaluated in all groups. Finally, in Exp 4, 39 estrous cycles of 11 mares were inseminated with unprocessed (n = 6) cooled-stored semen or semen cooled at 5 °C/24h and then processed by SpermFilter and resuspended in SM (n = 5), EY-C (n = 11), EY-nC (n = 11), or centrifuged and resuspended in EY-C (n = 6). Overall, semen processing and resuspension in EY mediums (EY-C and EY-nC) improved sperm parameters compared with those of unprocessed semen (P < 0.05). Centrifugation (91 ± 5 %) recovered more sperm than SpermFilter (84 ± 9 %; P < 0.05). Sperm resuspended in EY-nC maintained better sperm parameters throughout the thermoresistance test than those in the other groups (P < 0.05). The fertility rates were similar between all groups (P > 0.05). In conclusion, processing and resuspension in EY medium can improve sperm parameters in post-cooled-stored stallion semen.
Competing Interests: Declaration of competing interest A number of products used in this study are proprietary (Botupharma) and Drs. Marco A. Alvarenga and Frederico O. Papa are co-owners of Botupharma.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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