Neutrophils and monocyte toll like receptors 2 and 4 expression in preterm versus term delivery.

Autor:	Zahran AM; Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt., Zahran KM; Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University, Assiut, Egypt., Hetta HF; Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt., Badawy ER; Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt., Zahran ZAM; Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt., Ahmed YA; Department of Obstetrics and Gynecology, Faculty of Medicine, Suez University, P.O. Box 43221, Suez, Egypt., Shaltout AS; Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Jazyk:	angličtina
Zdroj:	The Egyptian journal of immunology [Egypt J Immunol] 2024 Jul; Vol. 31 (3), pp. 161-169.
Abstrakt:	Pregnancy results in an increase in immune cells, especially monocytes, which enhances the innate immune system. The increase of inflammatory cytokines in pregnant women's amniotic fluid, can cause uterine contraction, is linked to preterm labor. These inflammatory responses are controlled by Toll-like receptors (TLRs), which are largely expressed on neutrophils and monocytes. This study aimed to determine the role of neutrophils and monocyte subsets, as well as their expression of TLR-2 and TLR-4 in women with preterm and full-term delivery. The study involved a total of 74 women, comprising of 29 preterm labor, 25 full-term labor, and 20 non-pregnant women. The distribution of three monocyte subsets, namely (CD14++CD16-), (CD14+CD16+), and (CD14-/dim CD16++) was measured. Also, the expression of TLR2 and TLR4 in monocytes and neutrophils was analyzed using flow cytometry. Non-classical monocytes and intermediate monocytes were significantly higher in the preterm group than the control and full-term groups (p=0.041, p=0.043, and p=0.004, p= 0.049, respectively). Women in the preterm group showed significantly TLR2 expression on nonclassical monocytes compared to the control and full-term groups (p=0.002, and p=0.010, respectively). Also, preterm group expression of TLR4 was significantly higher in classical monocytes and nonclassical monocytes in comparison to the control group (p=0.019, and p≤0.0001, respectively). Besides, TLR4 expression was significantly up regulated in the preterm group compared to full-term in non-classical monocyte subset (p < 0.0001). Moreover, the expression of TLR-4 in neutrophils from the preterm group was statistically higher than expression from the full-term labor and control groups (p < .0001 for both). Such findings highlight the important role of monocyte subsets and neutrophils in activating the innate immune system and initiating strong pro-inflammatory responses that induce preterm labor. Additionally, TLR4 and TLR2 expressions on non-classical monocytes may be used as a marker to assess the probability of preterm labor. (Copyright© by the Egyptian Association of Immunologists.)
Databáze:	MEDLINE
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