Identification of a multi-modal mechanism for Se(VI) reduction and Se(0) allotropic transition by Stenotrophomonas bentonitica.

Autor: Ruiz-Fresneda MA; Department of Microbiology, University of Granada, Campus Fuentenueva, 18071, Granada, Spain. mafres@ugr.es., Lazúen-López G; Department of Microbiology, University of Granada, Campus Fuentenueva, 18071, Granada, Spain., Pérez-Muelas E; Department of Microbiology, University of Granada, Campus Fuentenueva, 18071, Granada, Spain., Peña-Martín J; Department of Human Anatomy and Embryology, Faculty of Medicine, University of Granada, 18016, Granada, Spain.; Centre for Biomedical Research (CIBM), Biopathology and Regenerative Medicine Institute (IBIMER), University of Granada, 18100, Granada, Spain., Linares-Jiménez RE; Department of Microbiology, University of Granada, Campus Fuentenueva, 18071, Granada, Spain.; Institute of Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf, Dresden, Germany., Newman-Portela AM; Department of Microbiology, University of Granada, Campus Fuentenueva, 18071, Granada, Spain., Merroun ML; Department of Microbiology, University of Granada, Campus Fuentenueva, 18071, Granada, Spain.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2024 Jul 12. Date of Electronic Publication: 2024 Jul 12.
DOI: 10.1007/s11356-024-34256-z
Abstrakt: Microorganisms can play a key role in selenium (Se) bioremediation and the fabrication of Se-based nanomaterials by reducing toxic forms (Se(VI) and Se(IV)) into Se(0). In recent years, omics have become a useful tool in understanding the metabolic pathways involved in the reduction process. This paper aims to elucidate the specific molecular mechanisms involved in Se(VI) reduction by the bacterium Stenotrophomonas bentonitica. Both cytoplasmic and membrane fractions were able to reduce Se(VI) to Se(0) nanoparticles (NPs) with different morphologies (nanospheres and nanorods) and allotropes (amorphous, monoclinic, and trigonal). Proteomic analyses indicated an adaptive response against Se(VI) through the alteration of several metabolic pathways including those related to energy acquisition, synthesis of proteins and nucleic acids, and transport systems. Whilst the thioredoxin system and the Painter reactions were identified to play a crucial role in Se reduction, flagellin may also be involved in the allotropic transformation of Se. These findings suggest a multi-modal reduction mechanism is involved, providing new insights for developing novel strategies in bioremediation and nanoparticle synthesis for the recovery of critical materials within the concept of circular economy.
(© 2024. The Author(s).)
Databáze: MEDLINE
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