RNA thermometers are widespread upstream of ABC transporter genes in bacteria.

Autor:	Tong AY; Department of Biology, California Lutheran University, Thousand Oaks, California, USA., Tong EL; Department of Biology, California Lutheran University, Thousand Oaks, California, USA., Hannani MA; Department of Biology, California Lutheran University, Thousand Oaks, California, USA., Shaffer SN; Laboratory of Nucleic Acids, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA., Santiago D; Department of Biology, California Lutheran University, Thousand Oaks, California, USA., Ferré-D'Amaré AR; Laboratory of Nucleic Acids, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA., Passalacqua LFM; Laboratory of Nucleic Acids, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA. Electronic address: luiz.passalacqua@nih.gov., Abdelsayed MM; Department of Biology, California Lutheran University, Thousand Oaks, California, USA. Electronic address: mabdelsayed@callutheran.edu.
Jazyk:	angličtina
Zdroj:	The Journal of biological chemistry [J Biol Chem] 2024 Aug; Vol. 300 (8), pp. 107547. Date of Electronic Publication: 2024 Jul 09.
DOI:	10.1016/j.jbc.2024.107547
Abstrakt:	RNA thermometers are temperature-sensing non-coding RNAs that regulate the expression of downstream genes. A well-characterized RNA thermometer motif discovered in bacteria is the ROSE-like element (repression of heat shock gene expression). ATP-binding cassette (ABC) transporters are a superfamily of transmembrane proteins that harness ATP hydrolysis to facilitate the export and import of substrates across cellular membranes. Through structure-guided bioinformatics, we discovered that ROSE-like RNA thermometers are widespread upstream of ABC transporter genes in bacteria. X-ray crystallography, biochemistry, and cellular assays indicate that these RNA thermometers are functional regulatory elements. This study expands the known biological role of RNA thermometers to these key membrane transporters. Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu