Proteomic profile of seminal plasma from Pêga donkeys (Equus asinus) with high sperm motility and vigor: Implications for assisted reproduction.

Autor: Costa ICTA; Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil. Electronic address: isabella.costa@ufv.br., Ramírez-López CJ; Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil; Department of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil. Electronic address: camilo.lopez@ufv.br., de Sousa WV; Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil; Department of Biochemistry, Institute of Chemistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. Electronic address: wassali@ufrj.br., da Silva YM; Department of Biochemistry, Institute of Chemistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. Electronic address: yaraMdS@ufrj.br., Villadiego FAC; Department of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Nogueira FCS; Department of Biochemistry, Institute of Chemistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. Electronic address: fabiocsn@iq.ufrj.br., Guimarães SEF; Department of Zootechnics, Universidade Federal de Viçosa, Viçosa, MG, Brazil. Electronic address: sfacioni@ufv.br., Guimarães JD; Department of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil. Electronic address: jdguima@ufv.br., Baracat-Pereira MC; Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, MG, Brazil. Electronic address: baracat@ufv.br.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part D, Genomics & proteomics [Comp Biochem Physiol Part D Genomics Proteomics] 2024 Jun 27; Vol. 52, pp. 101264. Date of Electronic Publication: 2024 Jun 27.
DOI: 10.1016/j.cbd.2024.101264
Abstrakt: Donkeys of the Pêga breed (Equus asinus) have been used for two centuries to produce breeding stock and create hybrids for labor and transport in southeast Brazil, and for exporting meat and milk to other countries. Furthermore, they are used in competitions, as they are docile and easy to handle. However, assisted reproduction success rates for frozen donkey semen are remarkably low, with no standardized method for cryopreserving sperm after removal of seminal plasma. This work aims to reveal the biological involvement of seminal plasma proteins from Pêga donkeys in aiding the development of assisted reproduction. This study was carried out with 14 ejaculates collected every eight days, throughout the breeding season, from three healthy fertile Pêga donkeys, with an average age of four years. After confirming the high freezability of fresh semen by evaluating quality parameters, the seminal plasma was separated by centrifugation and an aliquot from each collection was microfiltered and frozen. A label-free technique followed by LC-MS/MS analysis applied to pools of seminal plasma samples from each animal revealed 522 proteins in the proteomic profile, of which 49.8 % (260 proteins) are related to cellular energy transformation, and many proteins involved in reproduction (76), spermatogenesis (38), fertilization (29), among other biological process. By comparison with literature, Pêga donkeys share many proteins with donkeys of Dezhou breed that present great potential as fertility biomarkers. Our results showed proteins positively related to fertilization for different breeds of donkeys around the world, helping to enhance the assisted reproduction of Pêga donkeys.
Competing Interests: Declaration of competing interest The authors have nothing to disclose as conflict of interest.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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