Histopathological findings and diagnosis of cutaneous leishmaniasis, confirmed by PCR, in an endemic region of Brazil.

Autor: Pena HP; Federal University of São João Del-Rei, Divinópolis, MG, Brazil., Belo VS; Federal University of São João Del-Rei, Divinópolis, MG, Brazil., Fontes ID; University Center of Belo Horizonte - UniBH, Belo Horizonte, MG, Brazil., De Melo SN; Federal University of São João Del-Rei, Divinópolis, MG, Brazil., Soares PH; Federal University of São João Del-Rei, Divinópolis, MG, Brazil., Santos IM; Federal University of São João Del-Rei, Divinópolis, MG, Brazil., Leitão TJ; Federal University of São João Del-Rei, Divinópolis, MG, Brazil., Costa MZD; Laboratory of Cytology and Pathology, Divinópolis, MG, Brazil., Teixeira-Neto RG; Federal University of São João Del-Rei, Divinópolis, MG, Brazil., Da Silva ES; Federal University of São João Del-Rei, Divinópolis, MG, Brazil., Xavier-Junior JCC; Salesian Auxilium Catholic University Center (Unisalesiano), Araçatuba, SP, Brazil.
Jazyk: angličtina
Zdroj: Journal of infection in developing countries [J Infect Dev Ctries] 2024 Jun 30; Vol. 18 (6), pp. 895-899. Date of Electronic Publication: 2024 Jun 30.
DOI: 10.3855/jidc.18120
Abstrakt: Introduction: Diagnosis of cutaneous leishmaniasis (CL) is difficult, and the correct use of histopathological criteria can be useful in clinical practice. The present study evaluates the association between histopathological findings and the results of polymerase chain reaction (PCR) in clinically suspected cases of CL.
Methodology: Skin samples were received in a laboratory from an endemic region of Brazil for over nine years. Associations were analyzed by means of the Chi square test with a 5% level of significance.
Results: Of the 222 examined samples, 190 (85.6%) tested positive by PCR. All 25 cases identified by microscopic examination also tested positive by PCR. Except for the more intense inflammatory infiltrate, all other evaluated histological variables (ulceration, epidermal hyperplasia, hyperkeratosis, presence of granuloma, neutrophils, histiocytes, lymphocytes, plasmocytes, and necrosis) were not significantly associated with PCR positivity.
Conclusions: The intensity of the inflammatory infiltrate is a good indicator of the occurrence of CL. Histopathological aspects are useful to increase the predictive values of CL diagnoses, but PCR is still necessary to confirm or exclude the disease.
Competing Interests: No Conflict of Interest is declared
(Copyright (c) 2024 Heber P Pena, Vinícius S Belo, Igor dC Fontes, Saulo N de Melo, Paulo HA Soares, Ingrid M Santos, Tiago J Leitão, Maria Zélia dO Costa, Rafael G Teixeira-Neto, Eduardo S da Silva, José Cândido C Xavier-Junior.)
Databáze: MEDLINE
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