miR-126a-5p inhibits H1N1-induced inflammation and matrix protease secretion in lung fibroblasts by targeting ADAMTS-4.

Autor: Fang F; The 2nd department of Critical Care Medicine, Xi'an Chest Hospital, Hangtian Avenue East Section, Chang'an District, Xi'an, Shaanxi, 710100, China., Wang B; The 2nd department of Critical Care Medicine, Xi'an Chest Hospital, Hangtian Avenue East Section, Chang'an District, Xi'an, Shaanxi, 710100, China., Lu X; The 2nd department of Critical Care Medicine, Xi'an Chest Hospital, Hangtian Avenue East Section, Chang'an District, Xi'an, Shaanxi, 710100, China., Wang L; The 2nd department of Critical Care Medicine, Xi'an Chest Hospital, Hangtian Avenue East Section, Chang'an District, Xi'an, Shaanxi, 710100, China., Chen X; The 2nd department of Critical Care Medicine, Xi'an Chest Hospital, Hangtian Avenue East Section, Chang'an District, Xi'an, Shaanxi, 710100, China., Wang G; The 2nd department of Critical Care Medicine, Xi'an Chest Hospital, Hangtian Avenue East Section, Chang'an District, Xi'an, Shaanxi, 710100, China., Yang Y; The 2nd department of Critical Care Medicine, Xi'an Chest Hospital, Hangtian Avenue East Section, Chang'an District, Xi'an, Shaanxi, 710100, China. yifanyayy@163.com.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2024 Jul 11; Vol. 169 (8), pp. 164. Date of Electronic Publication: 2024 Jul 11.
DOI: 10.1007/s00705-024-06086-4
Abstrakt: Upregulation of ADAMTS-4 has been reported to have an important role in lung injury, and ADAMTS-4 expression is regulated by miR-126a-5p in abdominal aortic aneurysms. The aim of this study was to investigate whether miR-126a-5p/ADAMTS-4 plays a role in influenza-virus-induced lung injury. Lung fibroblasts were infected with H1N1 influenza virus to detect changes in miR-126a-5p and ADAMTS-4 expression, and cell viability was measured by CCK-8 assay. Inflammatory factors and matrix protease levels were examined using ELISA kits, and cell apoptosis was assessed by measuring the levels of apoptosis-related proteins. A dual luciferase assay was used to verify the regulatory relationship between miR-126a-5p and ADAMTS-4. H1N1 influenza virus reduced fibroblast viability, inhibited miR-126a-5p expression, and promoted ADAMTS-4 expression. Overexpression of miR-126a-5p attenuated the cellular inflammatory response, apoptosis, matrix protease secretion, and virus replication. Luciferase reporter assays revealed that miR-126a-5p inhibited ADAMTS-4 expression by targeting ADAMTS-4 mRNA. Further experiments showed that overexpression of ADAMTS-4 significantly reversed the inhibitory effects of miR-126a-5p on fibroblast inflammation, apoptosis, matrix protease secretion, and virus replication. Upregulation of miR-126a-5p inhibits H1N1-induced apoptosis, inflammatory factors, and matrix protease secretion, as well as virus replication in lung fibroblasts.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: