Preparing research samples for safe arrival at centers and facilities: recipes for successful experiments.

Autor: Bowman SEJ; Hauptman-Woodward Medical Research Institute, Buffalo, NY 14203, USA., Byrnes J; National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY 11973, USA., Russi S; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Zimanyi CM; National Center for CryoEM Access and Training, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY 10027, USA.
Jazyk: angličtina
Zdroj: Acta crystallographica. Section F, Structural biology communications [Acta Crystallogr F Struct Biol Commun] 2024 Aug 01; Vol. 80 (Pt 8), pp. 165-172. Date of Electronic Publication: 2024 Jul 11.
DOI: 10.1107/S2053230X24006174
Abstrakt: Preparation of biomacromolecules for structural biology studies is a complex and time-consuming process. The goal is to produce a highly concentrated, highly pure product that is often shipped to large facilities with tools to prepare the samples for crystallization trials or for measurements at synchrotrons and cryoEM centers. The aim of this article is to provide guidance and to discuss general considerations for shipping biomacromolecular samples. Details are also provided about shipping samples for specific experiment types, including solution- and cryogenic-based techniques. These guidelines are provided with the hope that the time and energy invested in sample preparation is not lost due to shipping logistics.
(open access.)
Databáze: MEDLINE
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