Unveiling the potential: Extracellular vesicles from plant cell suspension cultures as a promising source.

Autor: Kırbaş OK; Faculty of Engineering, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey., Sağraç D; Faculty of Engineering, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey., Çiftçi ÖC; Faculty of Engineering, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey., Özdemir G; Faculty of Engineering, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey., Öztürkoğlu D; Faculty of Engineering, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey., Bozkurt BT; Faculty of Engineering, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey., Derman ÜC; Faculty of Engineering, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey., Taşkan E; Faculty of Engineering, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey., Taşlı PN; Faculty of Engineering, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey., Özdemir BS; Faculty of Engineering, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey., Şahin F; Faculty of Engineering, Department of Genetics and Bioengineering, Yeditepe University, Istanbul, Turkey.
Jazyk: angličtina
Zdroj: BioFactors (Oxford, England) [Biofactors] 2024 Jul 11. Date of Electronic Publication: 2024 Jul 11.
DOI: 10.1002/biof.2090
Abstrakt: Extracellular vesicles are secreted by all eukaryotic cells and they have an important role in intercellular signaling. Plant extracellular vesicles (PEVs) are a novel area of research that has gained attention due to their potential implications in biomolecule transport and therapeutic applications. PEVs are lipid bilayer-enclosed structures that contain a diverse cargo of biomolecules such as proteins and lipids. Moreover, it is known that PEVs have a noticeable therapeutic potential for various conditions such as inflammation and oxidative stress. However, there are critical problems such as removing the endosomes and plant-derived biomolecules that decrease the standardization and therapeutic efficacy of PEVs. In our study, the aim was to characterize plant cell suspension-derived extracellular vesicles (PCSEVs) obtained from two different plant cell suspension cultures: Stevia rebaudiana and Vaccaria hispanica. These vesicles were isolated using ultrafiltration and characterized with nanoparticle tracking analysis (NTA) and atomic force microscopy (AFM). The molecular composition of PCSEVs was profiled and the cellular uptake assay was performed. Our results demonstrated that PCSEVs have a spherical shape, less than 200 nm. In the fatty acid analysis, the primary components in PCSEVs were palmitic acid, linoleic acid, and cis-vaccenic acid. The protein content of Stevia rebaudiana-derived EVs (SDEVs) was largely associated with proteins involved in extracellular structures and functions. Conversely, Vaccaria hispanica-derived EVs (HDEVs) displayed a higher presence of cytosolic proteins. These findings contribute to the understanding of PCSEVs and open up potential avenues in extracellular vesicle research, pointing to promising prospects for future innovations in various fields.
(© 2024 The Author(s). BioFactors published by Wiley Periodicals LLC on behalf of International Union of Biochemistry and Molecular Biology.)
Databáze: MEDLINE
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