Molecular Detection of Coxiella burnetii in Vaginal Swab Samples from Sheep That Aborted.

Autor:	Sánchez-Rodríguez O; Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, México., Palomares-Resendiz EG; CENID Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Carretera Federal México-Toluca, Cuajimalpa, México., Díaz-Aparicio E; CENID Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Carretera Federal México-Toluca, Cuajimalpa, México., Arellano-Reynoso B; Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, México., Martínez-Chavarría LC; Departamento de Patología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, México., Martínez-Serrano G; Departamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, México., Hernández-Castro R; Departamento de Ecología de Agentes Patógenos, Hospital General Dr. Manuel Gea González, Tlalpan, México.
Jazyk:	angličtina
Zdroj:	Vector borne and zoonotic diseases (Larchmont, N.Y.) [Vector Borne Zoonotic Dis] 2024 Dec; Vol. 24 (12), pp. 838-841. Date of Electronic Publication: 2024 Jul 11.
DOI:	10.1089/vbz.2023.0153
Abstrakt:	Background: Coxiella burnetii , an obligate intracellular bacterium, is the etiological agent of Q fever in humans and one of the causes of abortion in small ruminants. Although coxiellosis is considered an exotic disease, there are a few reports in Mexico. Methods: The objective of this work was to determine the presence of C . burnetii DNA in vaginal samples from sheep that presented abortion and ram semen. A total of 180 vaginal exudate samples and 20 semen samples were obtained from five Central and Southern States of Mexico. Total DNA was extracted from vaginal swabs and C . burnetii was identified by PCR amplification and sequencing of the IS 1111 insertion sequence. Results and Conclusion: In total, 110 (110/180) vaginal samples and 12 (12/20) semen samples were positive for C . burnetii . This is the first report of C . burnetii in sheep that aborted and in ram semen in Mexico.
Databáze:	MEDLINE
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