Human lymph node fibroblastic reticular cells maintain heterogeneous characteristics in culture.

Autor:	Roet JEG; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology & Immunology, De Boelelaan 1117, Amsterdam, the Netherlands.; Amsterdam Institute for Immunology and Infectious diseases, Amsterdam, the Netherlands., Morrison AI; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology & Immunology, De Boelelaan 1117, Amsterdam, the Netherlands.; Amsterdam Institute for Immunology and Infectious diseases, Amsterdam, the Netherlands., Mikula AM; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology & Immunology, De Boelelaan 1117, Amsterdam, the Netherlands.; Amsterdam Institute for Immunology and Infectious diseases, Amsterdam, the Netherlands.; Cancer Center Amsterdam, Cancer Biology & Immunology, Amsterdam, the Netherlands., de Kok M; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology & Immunology, De Boelelaan 1117, Amsterdam, the Netherlands.; Amsterdam Institute for Immunology and Infectious diseases, Amsterdam, the Netherlands., Panocha D; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology & Immunology, De Boelelaan 1117, Amsterdam, the Netherlands.; Amsterdam Institute for Immunology and Infectious diseases, Amsterdam, the Netherlands.; Cancer Center Amsterdam, Cancer Biology & Immunology, Amsterdam, the Netherlands., Roest HP; Erasmus MC Transplant Institute, University Medical Center Rotterdam, Department of Surgery, Dr. Molewaterplein 40, 3015 GD Rotterdam, the Netherlands., van der Laan LJW; Erasmus MC Transplant Institute, University Medical Center Rotterdam, Department of Surgery, Dr. Molewaterplein 40, 3015 GD Rotterdam, the Netherlands., de Winde CM; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology & Immunology, De Boelelaan 1117, Amsterdam, the Netherlands.; Amsterdam Institute for Immunology and Infectious diseases, Amsterdam, the Netherlands.; Cancer Center Amsterdam, Cancer Biology & Immunology, Amsterdam, the Netherlands., Mebius RE; Amsterdam UMC location Vrije Universiteit Amsterdam, Molecular Cell Biology & Immunology, De Boelelaan 1117, Amsterdam, the Netherlands.; Amsterdam Institute for Immunology and Infectious diseases, Amsterdam, the Netherlands.; Cancer Center Amsterdam, Cancer Biology & Immunology, Amsterdam, the Netherlands.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2024 Jun 04; Vol. 27 (7), pp. 110179. Date of Electronic Publication: 2024 Jun 04 (Print Publication: 2024).
DOI:	10.1016/j.isci.2024.110179
Abstrakt:	Fibroblastic reticular cells (FRCs) are mesenchymal stromal cells in human lymph nodes (LNs) playing a pivotal role in adaptive immunity. Several FRC subsets have been identified, yet it remains to be elucidated if their heterogeneity is maintained upon culture. Here, we established a protocol to preserve and culture FRCs from human LNs and characterized their phenotypic profile in fresh LN suspensions and upon culture using multispectral flow cytometry. We found nine FRC subsets in fresh human LNs, independent of donor, of which four persisted in culture throughout several passages. Interestingly, the historically FRC-defining marker podoplanin (PDPN) was not present on all FRC subsets. Therefore, we propose that CD45 neg CD31 neg human FRCs are not restricted by PDPN expression, as we found CD90, BST1, and CD146/MCAM to be more widely expressed. Together, our data provide insight into FRC heterogeneity in human LNs, enabling further investigation into the function of individual FRC subsets. Competing Interests: The authors declare no competing interests. (© 2024 The Author(s).)
Databáze:	MEDLINE
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