Ultrasonic high-yield extraction of non-toxic fucose-containing Abroma augusta polysaccharide bearing emulsifying properties.

Autor: Sarkhel S; Laboratory of Applied Food Chemistry, Microbiology, and Process Engineering, Centre for Food Engineering and Technology, Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, India., Mondal M; Membrane Science and Separation Technology Division, GB Marg, CSIR - Central Salt and Marine Chemicals Research Institute, Bhavnagar, India., Datta D; Laboratory of Applied Food Chemistry, Microbiology, and Process Engineering, Centre for Food Engineering and Technology, Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, India., Sahoo B; Laboratory of Applied Food Chemistry, Microbiology, and Process Engineering, Centre for Food Engineering and Technology, Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, India., Kumari A; Laboratory of Applied Food Chemistry, Microbiology, and Process Engineering, Centre for Food Engineering and Technology, Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, India., Saha S; Laboratory of Applied Food Chemistry, Microbiology, and Process Engineering, Centre for Food Engineering and Technology, Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, India., Bera S; Laboratory of Applied Food Chemistry, Microbiology, and Process Engineering, Centre for Food Engineering and Technology, Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, India., Jana M; Department of Neurological Sciences, Rush University Medical Center, Chicago, IL, USA., Tiwari A; Laboratory of Applied Food Chemistry, Microbiology, and Process Engineering, Centre for Food Engineering and Technology, Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, India., Roy A; Laboratory of Applied Food Chemistry, Microbiology, and Process Engineering, Centre for Food Engineering and Technology, Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, India.
Jazyk: angličtina
Zdroj: Journal of the science of food and agriculture [J Sci Food Agric] 2024 Nov; Vol. 104 (14), pp. 8858-8868. Date of Electronic Publication: 2024 Jul 11.
DOI: 10.1002/jsfa.13712
Abstrakt: Background: The stem of Abroma augusta contains mucilaginous polysaccharides having numerous ethnomedicinal properties. The present work aimed to develop a scalable ultrasonic-assisted aqueous Abroma augusta mucilage (AAM) extraction (UAE) method and further explores its emulsifying property and toxicity concern.
Results: The combination of ultrasonic power (750 W), solid-to-liquid ratio (1:15) and temperature (348 K) gave the highest extraction yield of 2.28% with a diffusivity value of 3.85 × 10 -9  m 2  s -1 , which was higher than aqueous extraction method using a kinetic model based on Fick's second law of diffusion. The extracted polysaccharide showed no toxicity as measured through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assay on RAW cell line. Additionally, the polysaccharide over its critical micelle concentration (400, 500, 600 and 700 μg mL -1 ) offered emulsifying properties with 0.5%, 1% and 5% oil (v/v). The emulsion with a polysaccharide concentration of 600 μg mL -1 with 5% oil (v/v) provides stability against coalescence for 3 days.
Conclusion: The overall findings indicated that UAE of AAM polysaccharide can be used for an efficient extraction method, and the obtained polysaccharide is nontoxic in nature and bears emulsifying properties. © 2024 Society of Chemical Industry.
(© 2024 Society of Chemical Industry.)
Databáze: MEDLINE
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