Isolation and characterisation of two epithelial-like cell lines from the gills of Chrysophrys auratus (Australasian snapper) and Oncorhynchus tshawytscha (Chinook salmon) and their use in aquatic toxicology.

Autor: Böhmert B; The New Zealand Institute for Plant and Food Research Limited, Nelson Research Centre, 293 Akersten Street, Nelson, 7010, New Zealand., Chong GLW; The New Zealand Institute for Plant and Food Research Limited, Nelson Research Centre, 293 Akersten Street, Nelson, 7010, New Zealand., Lo K; The New Zealand Institute for Plant and Food Research Limited, Mt Albert Research Centre, Auckland, 1142, New Zealand., Algie M; The New Zealand Institute for Plant and Food Research Limited, Nelson Research Centre, 293 Akersten Street, Nelson, 7010, New Zealand., Colbert D; The New Zealand Institute for Plant and Food Research Limited, Mt Albert Research Centre, Auckland, 1142, New Zealand., Jordan MD; The New Zealand Institute for Plant and Food Research Limited, Mt Albert Research Centre, Auckland, 1142, New Zealand., Stuart G; Department of Pharmacology and Toxicology, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand., Wise LM; Department of Pharmacology and Toxicology, School of Biomedical Sciences, University of Otago, Dunedin, New Zealand., Lee LEJ; Faculty of Science, University of the Fraser Valley, Abbotsford, BC, V2S 7M8, Canada., Bols NC; Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada., Dowd GC; The New Zealand Institute for Plant and Food Research Limited, Nelson Research Centre, 293 Akersten Street, Nelson, 7010, New Zealand. Georgina.Dowd@plantandfood.co.nz.
Jazyk: angličtina
Zdroj: In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 2024 Jul 10. Date of Electronic Publication: 2024 Jul 10.
DOI: 10.1007/s11626-024-00941-z
Abstrakt: In vitro gill models are becoming increasingly important in aquatic toxicology, yet the fish gill invitrome is underrepresented, encompassing approximately 0.1% of extant species. Here, we describe the establishment and characterisation of two gill-derived, epithelial-like cell lines isolated from fish species of significant importance to New Zealand: Chrysophrys auratus (Australasian snapper) and Oncorhynchus tshawytscha (Chinook salmon). Designated CAgill1PFR (Chrysophrys auratus, gill 1, Plant & Food Research) and OTgill1PFR (Oncorhynchus tshawytscha, gill 1, Plant & Food Research), these cell lines have each been passaged greater than each 70 times over several years and are considered spontaneously immortalised. Both cell lines required serum for growth and exhibited differential responses to basal media formulations. CAgill1PFR was sensitive to low temperatures (4 °C) but replicated at high temperatures (30 °C), whereas OTgill1PFR was sensitive to high temperatures but remained viable at low temperatures, mirroring the natural environment of their host species. Immunostaining revealed expression of epithelial cell markers cytokeratin and E-cadherin, alongside positivity for the mesenchymal cell marker, vimentin. CAgill1PFR was more sensitive to the environmental toxin 3,4 dichloroaniline than OTgill1PFR through measurements of metabolic activity, membrane integrity, and lysosomal function. Furthermore, CAgill1PFR produced less CYP1A activity, indicative of ongoing biotransformation processes, in response to beta-naphthoflavone than OTgill1PFR. These cell lines expand the toolbox of resources and emphasise the need for species-specific aquatic toxicology research.
(© 2024. The Author(s).)
Databáze: MEDLINE
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