Inhibitory effect of polyphenols from sumac, pomegranate and Indian almond on urease producing bacteria and jack bean urease activity.

Autor: Haq MA; Department of Food Science & Technology, University of Karachi, 75270, Pakistan. Electronic address: abdul.haq@uok.edu.pk., Nazir M; Department of Food Science & Technology, University of Karachi, 75270, Pakistan., Jabeen G; Department of Microbiology, University of Karachi, 75270, Pakistan., Jabeen N; Department of Microbiology, University of Karachi, 75270, Pakistan. Electronic address: nusrat.jabeen@uok.edu.pk., Naz S; Department of Food Science & Technology, University of Karachi, 75270, Pakistan. Electronic address: shahina.naz@uok.edu.pk., Nawaz H; Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, School of Chemistry and Chemical Engineering, Huaiyin Normal University, Changjiangxi Road, Huaian 223300, Jiangsu, PR China. Electronic address: 8202311006@hytc.edu.cn., Xu J; Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, School of Chemistry and Chemical Engineering, Huaiyin Normal University, Changjiangxi Road, Huaian 223300, Jiangsu, PR China. Electronic address: xujiaxing@hytc.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Sep; Vol. 276 (Pt 1), pp. 133735. Date of Electronic Publication: 2024 Jul 08.
DOI: 10.1016/j.ijbiomac.2024.133735
Abstrakt: Urinary tract infection caused by Klebsiella, Proteus and Streptococcus is a urease dependent process, hence treatment of these infections by antibacterial compounds lies in inhibition of their virulence factors. The crude methanolic extracts derived from sumac fruit, pomegranate peel and Indian almond leaves were separated into anthocyanin and non-anthocyanin fractions using solid phase cartridges. The inhibitory effect of these fractions was determined on the growth of urease producing species and jack bean urease activity. Known compounds in the fractions were also docked with ureases of different biological origins viz. K. pneumoniae (PDB ID: 8HCN), K. aerogenes (PDB ID: 2KAU), Helicobacter pylori (PDB ID:8HC1)and Canavalia ensiformis (jack bean) (PDB ID: 3LA4) to determine their binding affinities and interaction with the enzyme. All the fractions showed significant inhibition growth for P. mirabilis, S. epidermidis and K.pneumoniae. Among the samples, sumac showed greatest inhibition against all (MIC 6-25 mg.mL -1 ) while among the fractions, anthocyanin was found to be most active (MIC 6-12 mg/mL). Likewise, all fractions inhibited urease with lowest ICs 50 shown by sumac fractions (21-116 μg.mL -1 ). Out of 39 compounds docked, 27 showed interaction with movable flaps and/or active site of ureases which explains their mode of inhibition.
Competing Interests: Declaration of competing interest This is to be certified that there is no conflict of interest in the submission of research paper entitled “Inhibitory Effect of Polyphenols from Sumac, Pomegranate and Indian Almond on Urease Producing Bacteria and Jack Bean Urease Activity.”
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Databáze: MEDLINE
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