An Extended Flow Cytometry Evaluation of ex Vivo Expanded NK Cells Using K562.Clone1, a Feeder Cell Line Manufactured in Brazil.

Autor: Watanabe CM; Experimental Research Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil., Suzuki CI; Experimental Research Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil., Dos Santos AM; Experimental Research Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil., Aloia TPA; Experimental Research Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil., Lee G; Department of Pathology, Case Western Reserve University, Cleveland, Ohio., Wald D; Department of Pathology, Case Western Reserve University, Cleveland, Ohio; Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, Ohio., Okamoto OK; Department of Hemotherapy and Cellular Therapy, Hospital Israelita Albert Einstein, São Paulo, Brazil; Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, São Paulo, Brazil., de Azevedo JTC; Experimental Research Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil; Department of Hemotherapy and Cellular Therapy, Hospital Israelita Albert Einstein, São Paulo, Brazil., de Godoy JAP; Experimental Research Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil; Department of Hemotherapy and Cellular Therapy, Hospital Israelita Albert Einstein, São Paulo, Brazil., Santos FPS; Experimental Research Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil; Oncology and Hematology Center, Familia Dayan-Daycoval, Hospital Israelita Albert Einstein, São Paulo, Brazil., Weinlich R; Experimental Research Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil., Kerbauy LN; Experimental Research Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil; Department of Hemotherapy and Cellular Therapy, Hospital Israelita Albert Einstein, São Paulo, Brazil; Oncology and Hematology Center, Familia Dayan-Daycoval, Hospital Israelita Albert Einstein, São Paulo, Brazil., Kutner JM; Department of Hemotherapy and Cellular Therapy, Hospital Israelita Albert Einstein, São Paulo, Brazil; Oncology and Hematology Center, Familia Dayan-Daycoval, Hospital Israelita Albert Einstein, São Paulo, Brazil., Paiva RMA; Experimental Research Laboratory, Hospital Israelita Albert Einstein, São Paulo, Brazil; Department of Hemotherapy and Cellular Therapy, Hospital Israelita Albert Einstein, São Paulo, Brazil. Electronic address: raquel.paiva@einstein.br., Hamerschlak N; Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Transplantation and cellular therapy [Transplant Cell Ther] 2024 Nov; Vol. 30 (11), pp. 1063.e1-1063.e19. Date of Electronic Publication: 2024 Jul 08.
DOI: 10.1016/j.jtct.2024.07.004
Abstrakt: Natural killer (NK) cells play a crucial role in the immune system's response against cancer. However, the challenge of obtaining the required quantity of NK cells for effective therapeutic response necessitates the development of strategies for their ex vivo expansion. This study aimed to develop a novel feeder cell line, K562.Clone1, capable of promoting the ex vivo expansion of NK cells while preserving their cytotoxic potential. he K562 leukemic cell line was transduced with mbIL-21 and 4-1BBL proteins to generate K562.Clone1 cells. NK cells were then co-cultured with these feeder cells, and their expansion rate was monitored over 14 days. The cytotoxic potential of the expanded NK cells was evaluated against acute myeloid leukemia blasts and tumor cell lines of leukemia and glial origin. Statistical analysis was performed to determine the significance of the results. The K562.Clone1 co-cultured with peripheral NK showed a significant increase in cell count, with an approximate 94-fold expansion over 14 days. Expanded NK cells demonstrated cytotoxicity against the tested tumor cell lines, indicating preservation of their cytotoxic characteristics. Additionally, the CD56, CD16, inhibitory KIRs, and activation receptors were conserved and present in a well-balanced manner. The study successfully developed a feeder cell line, K562.Clone1, that effectively promotes the expansion of NK cells ex vivo while maintaining their cytotoxic potential. This development could significantly contribute to the advancement of NK cell therapy, especially in Brazil.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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