Graded or random - Effect of pore distribution in 3D titanium scaffolds on corrosion performance and response of hMSCs.

Autor: Idaszek J; Warsaw University of Technology, Faculty of Materials Science and Engineering, Warsaw, Poland. Electronic address: joanna.idaszek@pw.edu.pl., Wysocki B; Cardinal Stefan Wyszynski University in Warsaw, Multidisciplinary Research Center, Dziekanow Lesny, Poland., Ura-Bińczyk E; Warsaw University of Technology, Faculty of Materials Science and Engineering, Warsaw, Poland., Dobkowska A; Warsaw University of Technology, Faculty of Materials Science and Engineering, Warsaw, Poland., Nowak W; Cardinal Stefan Wyszynski University in Warsaw, Multidisciplinary Research Center, Dziekanow Lesny, Poland., Yamamoto A; National Institute for Materials Science, Research Center for Macromolecules and Biomaterials, Tsukuba, Japan., Sulka GD; Jagiellonian University, Faculty of Chemistry, Department of Physical Chemistry and Electrochemistry, Gronostajowa 2, 30387 Krakow, Poland., Święszkowski W; Warsaw University of Technology, Faculty of Materials Science and Engineering, Warsaw, Poland.
Jazyk: angličtina
Zdroj: Biomaterials advances [Biomater Adv] 2024 Oct; Vol. 163, pp. 213955. Date of Electronic Publication: 2024 Jul 08.
DOI: 10.1016/j.bioadv.2024.213955
Abstrakt: Researchers agree that the ideal scaffold for tissue engineering should possess a 3D and highly porous structure, biocompatibility to encourage cell/tissue growth, suitable surface chemistry for cell attachment and differentiation, and mechanical properties that match those of the surrounding tissues. However, there is no consensus on the optimal pore distribution. In this study, we investigated the effect of pore distribution on corrosion resistance and performance of human mesenchymal stem cells (hMSC) using titanium scaffolds fabricated by laser beam powder bed fusion (PBF-LB). We designed two scaffold architectures with the same porosities (i.e., 75 %) but different distribution of pores of three sizes (200, 500, and 700 μm). The pores were either grouped in three zones (graded, GRAD) or distributed randomly (random, RAND). Microfocus X-ray computed tomography revealed that the chemically polished scaffolds had the porosity of 69 ± 4 % (GRAD) and 71 ± 4 % (RAND), and that the GRAD architecture had the higher surface area (1580 ± 101 vs 991 ± 62 mm 2 ) and the thinner struts (221 ± 37 vs 286 ± 14 μm). The electrochemical measurements demonstrated that the apparent corrosion rate of chemically polished GRAD scaffold decreased with the immersion time extension, while that for polished RAND was increased. The RAND architecture outperformed the GRAD one with respect to hMSC proliferation (over two times higher although the GRAD scaffolds had 85 % higher initial cell retention) and migration from a monolayer. Our findings demonstrate that the pore distribution affects the biological properties of the titanium scaffolds for bone tissue engineering.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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