Tool to Resolve Distortions in Elemental and Isotopic Imaging.

Autor: Lu C; Department of Chemistry, The University of Hong Kong, Pok Fu Lam, Hong Kong 999077, P. R. China., Chen G; Department of Chemistry, The University of Hong Kong, Pok Fu Lam, Hong Kong 999077, P. R. China., Song W; Departments of Medicine, University of California, Los Angeles, California 90095, United States., Chen K; School of Molecular Sciences, University of Western Australia, Perth 6009, Australia., Hee C; School of Molecular Sciences, University of Western Australia, Perth 6009, Australia., Nikan M; Ionis Pharmaceuticals, Inc., Carlsbad, California 92010, United States., Guagliardo P; Centre for Microscopy, Characterisation and Analysis, University of Western Australia, Perth 6009, Australia., Bennett CF; Ionis Pharmaceuticals, Inc., Carlsbad, California 92010, United States., Seth P; Ionis Pharmaceuticals, Inc., Carlsbad, California 92010, United States., Iyer KS; School of Molecular Sciences, University of Western Australia, Perth 6009, Australia., Young SG; Departments of Medicine, University of California, Los Angeles, California 90095, United States.; Human Genetics, University of California, Los Angeles, California 90095, United States., Qi X; Electrical and Electronic Engineering, The University of Hong Kong, Pok Fu Lam, Hong Kong 999077, P. R. China., Jiang H; Department of Chemistry, The University of Hong Kong, Pok Fu Lam, Hong Kong 999077, P. R. China.
Jazyk: angličtina
Zdroj: Journal of the American Chemical Society [J Am Chem Soc] 2024 Jul 24; Vol. 146 (29), pp. 20221-20229. Date of Electronic Publication: 2024 Jul 10.
DOI: 10.1021/jacs.4c05384
Abstrakt: Nanoscale secondary ion mass spectrometry (NanoSIMS) makes it possible to visualize elements and isotopes in a wide range of samples at a high resolution. However, the fidelity and quality of NanoSIMS images often suffer from distortions because of a requirement to acquire and integrate multiple image frames. We developed an optical flow-based algorithm tool, NanoSIMS Stabilizer, for all-channel postacquisition registration of images. The NanoSIMS Stabilizer effectively deals with the distortions and artifacts, resulting in a high-resolution visualization of isotope and element distribution. It is open source with an easy-to-use ImageJ plugin and is accompanied by a Python version with GPU acceleration.
Databáze: MEDLINE