Physiological Adaptations to Progressive Endurance Exercise Training in Adult and Aged Rats: Insights from the Molecular Transducers of Physical Activity Consortium (MoTrPAC).

Autor: Schenk S; Department of Orthopaedic Surgery, School of Medicine, University of California San Diego, La Jolla, CA 92093, USA., Sagendorf TJ; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA., Many GM; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA., Lira AK; Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA., de Sousa LGO; Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.; Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA., Bae D; Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA., Cicha M; Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA., Kramer KS; Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA., Muehlbauer M; Duke Molecular Physiology Institute, Duke University Medical Center, Durham, NC 27701, USA., Hevener AL; Division of Endocrinology, Diabetes, and Hypertension, Department of Medicine, University of California, Los Angeles, CA 90095, USA., Rector RS; Research Service, Harry S. Truman Memorial Veterans' Medical Center, Columbia, MO 65201, USA.; NextGen Precision Health, University of Missouri, Columbia, MO 65201, USA.; Department of Nutrition and Exercise Physiology, University of Missouri, Columbia, MO 65211, USA.; Division of Gastroenterology and Hepatology, Department of Medicine, University of Missouri, Columbia, MO 65211, USA., Thyfault JP; Department of Cell Biology and Physiology, University of Kansas Medical Center, Kansas City, KS 66160, USA.; KU Diabetes Institute, University of Kansas Medical Center, Kansas City, KS 66160, USA., Williams JP; Division of Aging Biology, National Institute on Aging, National Institutes of Health, Bethesda, MD 20898, USA., Goodyear LJ; Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA., Esser KA; Department of Physiology and Aging, College of Medicine, University of Florida, Gainesville, FL 32610, USA., Newgard CB; Duke Molecular Physiology Institute, Duke University Medical Center, Durham, NC 27701, USA., Bodine SC; Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.; Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
Jazyk: angličtina
Zdroj: Function (Oxford, England) [Function (Oxf)] 2024 Jul 11; Vol. 5 (4).
DOI: 10.1093/function/zqae014
Abstrakt: While regular physical activity is a cornerstone of health, wellness, and vitality, the impact of endurance exercise training on molecular signaling within and across tissues remains to be delineated. The Molecular Transducers of Physical Activity Consortium (MoTrPAC) was established to characterize molecular networks underlying the adaptive response to exercise. Here, we describe the endurance exercise training studies undertaken by the Preclinical Animal Sites Studies component of MoTrPAC, in which we sought to develop and implement a standardized endurance exercise protocol in a large cohort of rats. To this end, Adult (6-mo) and Aged (18-mo) female (n = 151) and male (n = 143) Fischer 344 rats were subjected to progressive treadmill training (5 d/wk, ∼70%-75% VO2max) for 1, 2, 4, or 8 wk; sedentary rats were studied as the control group. A total of 18 solid tissues, as well as blood, plasma, and feces, were collected to establish a publicly accessible biorepository and for extensive omics-based analyses by MoTrPAC. Treadmill training was highly effective, with robust improvements in skeletal muscle citrate synthase activity in as little as 1-2 wk and improvements in maximum run speed and maximal oxygen uptake by 4-8 wk. For body mass and composition, notable age- and sex-dependent responses were observed. This work in mature, treadmill-trained rats represents the most comprehensive and publicly accessible tissue biorepository, to date, and provides an unprecedented resource for studying temporal-, sex-, and age-specific responses to endurance exercise training in a preclinical rat model.
(© The Author(s) 2024. Published by Oxford University Press on behalf of American Physiological Society.)
Databáze: MEDLINE
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