Optimizing the preparation of paraffin sections from stallion testes.

Autor:	Asgenbaatar N; Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot, China.; Da Bei Nong Group Rumination Technology Rumination Academy, Haidian District, Beijing, China., Yi M; Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot, China., Wang X; Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot, China.; Collaborative Innovation Center for Birth Defect Research and Transformation of Shandong Province, Jining Medical University, Jining, China., Ulaangerel T; Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot, China., Shen Y; Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot, China., Wen X; Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot, China., Du M; Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot, China., Dong X; Da Bei Nong Group Rumination Technology Rumination Academy, Haidian District, Beijing, China.; China Agricultural University, Beijing, China., Mengkh Y; Xilingol League Animal Husbandry Work Station, Inner Mongolia Xilinhot, China., Dugarjav M; Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot, China., Bou G; Inner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, Inner Mongolia Agricultural University, Hohhot, China. gerelchimeng@imau.edu.cn.
Jazyk:	angličtina
Zdroj:	Histology and histopathology [Histol Histopathol] 2024 Jun 06, pp. 18775. Date of Electronic Publication: 2024 Jun 06.
DOI:	10.14670/HH-18-775
Abstrakt:	The preparation of paraffin sections is an important experimental technique in animal histological research, and key factors that determine the quality of a section include the dehydration time, waxing time, and drying temperature of the paraffin section. Paraffin sections obtained from testis tissue of adult horses exhibited higher quality with clear tissue structure and complete cell morphology after they underwent gradient dehydration for 6 hours, were immersed in wax for 60 minutes, and were dried in a 75-degree oven for 15 minutes. The detailed, optimized procedures that are developed in the current study may simplify histological experiments and research on equine testes. (©The Author(s) 2024. Open Access. This article is licensed under a Creative Commons CC-BY International License.)
Databáze:	MEDLINE
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