Development of a rapid and reliable method to simultaneously detect seven food allergens in processed foods using LC-MS/MS.

Autor:	Torii A; Nisshin Seifun Group Inc., 5-3-1 Tsurugaoka, Fujimino-City, Saitama 356-8511, Japan.; Hoshi University, School of Pharmacy and Pharmaceutical Sciences, Department of Analytical Chemistry, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan., Seki Y; Nisshin Seifun Group Inc., 5-3-1 Tsurugaoka, Fujimino-City, Saitama 356-8511, Japan., Sasano R; Hoshi University, School of Pharmacy and Pharmaceutical Sciences, Department of Analytical Chemistry, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.; AiSTI SCIENCE CO., Ltd., 18-3 Arimoto, Wakayama-City, Wakayama 640-8390, Japan., Ishida Y; Nisshin Seifun Group Inc., 5-3-1 Tsurugaoka, Fujimino-City, Saitama 356-8511, Japan., Nakamura K; National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki-City, Kanagawa 210-9501, Japan., Ito R; Hoshi University, School of Pharmacy and Pharmaceutical Sciences, Department of Analytical Chemistry, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan., Iwasaki Y; Hoshi University, School of Pharmacy and Pharmaceutical Sciences, Department of Analytical Chemistry, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan., Iijima K; Nisshin Seifun Group Inc., 5-3-1 Tsurugaoka, Fujimino-City, Saitama 356-8511, Japan., Akiyama H; Hoshi University, School of Pharmacy and Pharmaceutical Sciences, Department of Analytical Chemistry, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.; National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki-City, Kanagawa 210-9501, Japan.
Jazyk:	angličtina
Zdroj:	Food chemistry: X [Food Chem X] 2024 Jun 14; Vol. 23, pp. 101558. Date of Electronic Publication: 2024 Jun 14 (Print Publication: 2024).
DOI:	10.1016/j.fochx.2024.101558
Abstrakt:	Rapid analysis of multiple food allergens is required to confirm the appropriateness of food allergen labelling in processed foods. This study aimed to develop a rapid and reliable method to simultaneously detect trace amounts of seven food allergenic proteins (wheat, buckwheat, milk, egg, crustacean, peanut, and walnut) in processed foods using LC-MS/MS. Suspension-trapping (S-Trap) columns and on-line automated solid-phase extraction were used to improve the complex and time-consuming pretreatment process previously required for allergen analysis using LC-MS/MS. The developed method enabled the simultaneous detection of selected marker peptides for specific proteins derived from seven food ingredients in five types of incurred samples amended with trace amounts of allergenic proteins. The limit of detection values of the method for each protein were estimated to be <1 mg/kg. The developed analytical approach is considered an effective screening method for confirming food allergen labelling on a wide range of processed foods. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors. Published by Elsevier Ltd.)
Databáze:	MEDLINE
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