Therapeutic effects of Lingguizhugan decoction in a rat model of high-fat diet-induced insulin resistance.

Autor:	Liu XM; Department of Basic Medicine, School of Basic Medicine Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan Province, China., Yuan SQ; Department of Basic Medicine, School of Basic Medicine Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan Province, China., Ning Y; Department of Basic Medicine, School of Basic Medicine Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan Province, China., Nie SJ; Department of Basic Medicine, School of Basic Medicine Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan Province, China., Wang XQ; Department of Basic Medicine, School of Basic Medicine Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan Province, China., Jia HY; Department of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China., Zheng XL; Department of Basic Medicine, School of Basic Medicine Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan Province, China. zhengxl023@163.com.
Jazyk:	angličtina
Zdroj:	World journal of diabetes [World J Diabetes] 2024 Jun 15; Vol. 15 (6), pp. 1291-1298.
DOI:	10.4239/wjd.v15.i6.1291
Abstrakt:	Background: Lingguizhugan (LGZG) decoction is a widely used classic Chinese medicine formula that was recently shown to improve high-fat diet (HFD)-induced insulin resistance (IR) in animal studies. Aim: To assess the therapeutic effect of LGZG decoction on HFD-induced IR and explore the potential underlying mechanism. Methods: To establish an IR rat model, a 12-wk HFD was administered, followed by a 4-wk treatment with LGZG. The determination of IR status was achieved through the use of biochemical tests and oral glucose tolerance tests. Using a targeted meta-bolomics platform to analyze changes in serum metabolites, quantitative real-time PCR (qRT-PCR) was used to assess the gene expression of the ribosomal protein S6 kinase beta 1 (S6K1). Results: In IR rats, LGZG decreased body weight and indices of hepatic steatosis. It effectively controlled blood glucose and food intake while protecting islet cells. Metabolite analysis revealed significant differences between the HFD and HFD-LGZG groups. LGZG intervention reduced branched-chain amino acid levels. Levels of IR-related metabolites such as tryptophan, alanine, taurine, and asparagine decreased significantly. IR may be linked to amino acids due to the contemporaneous increase in S6K1 expression, as shown by qRT-PCR. Conclusions: Our study strongly suggests that LGZG decoction reduces HFD-induced IR. LGZG may activate S6K1 via metabolic pathways. These findings lay the groundwork for the potential of LGZG as an IR treatment. Competing Interests: Conflict-of-interest statement: Dr. Zhen has nothing to disclose. (©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu