A new diagnostic technique for identifying Angiostrongylus spp. larvae in intermediate snail species by examining the buccal cavity.

Autor: Zhao YB; Institute of Schistosomiasis Prevention and Control, Dali, 671000, Yunnan Province, China., Jiang L; Institute of Schistosomiasis Prevention and Control, Dali, 671000, Yunnan Province, China., Fang W; Institute of Schistosomiasis Prevention and Control, Dali, 671000, Yunnan Province, China., Chen SR; Institute of Schistosomiasis Prevention and Control, Dali, 671000, Yunnan Province, China., Liu YH; Institute of Schistosomiasis Prevention and Control, Dali, 671000, Yunnan Province, China., Zhao SH; Institute of Schistosomiasis Prevention and Control, Dali, 671000, Yunnan Province, China., Andrus PS; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, Key Laboratory on Parasite and Vector Biology, Ministry of Health, WHO Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China.; School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China., Li TM; Institute of Schistosomiasis Prevention and Control, Dali, 671000, Yunnan Province, China. litianmeisky@163.com., Guo YH; National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, Key Laboratory on Parasite and Vector Biology, Ministry of Health, WHO Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, 200025, China. guoyh@nipd.chinacdc.cn.
Jazyk: angličtina
Zdroj: Parasites & vectors [Parasit Vectors] 2024 Jul 09; Vol. 17 (1), pp. 298. Date of Electronic Publication: 2024 Jul 09.
DOI: 10.1186/s13071-024-06350-1
Abstrakt: Background: Angiostrongyliasis is a zoonotic parasitic disease caused by the rat lungworm Angiostrongylus cantonensis. The intermediate hosts of A. cantonensis are gastropods, and snail species such as Pomacea canaliculata play a key role in the transmission of human angiostrongyliasis. Detecting A. cantonensis infection in snails is an important component of epidemiological surveillance and the control of angiostrongyliasis.
Methods: In this study, a new method for diagnosing A. cantonensis infection in gastropods was developed by recovering larvae from the buccal cavity of three snail species. The entire buccal cavity of a snail was extracted, and the tissue was pressed between two microscope slides to observe whether A. cantonensis larvae were present. Our new method was compared with traditional pathogenic detection methods of lung microscopy, tissue homogenization, and artificial digestion. We artificially infected 160 P. canaliculata, 160 Cipangopaludina chinensis, and 160 Bellamya aeruginosa snails with A. cantonensis. Then, the four different detection methods were used to diagnose infection in each snail species at 7, 14, 21, and 28 days post exposure.
Results: We found no significant difference in the percentages of infected P. canaliculata snails using the four methods to detect A. cantonensis larvae. The radula pressing method had a mean detection rate of 80%, while the lung microscopy (81.3%), tissue homogenization (83.8%), and artificial digestion (85%) methods had slightly greater detection rates. Similarly, the percentages of infected C. chinensis snails that were detected using the radula pressing (80%), tissue homogenization (82.1%), and artificial digestion (83.8%) methods were not significantly different. Finally, the percentages of infected B. aeruginosa snails that were detected using the radula pressing (81.3%), tissue homogenization (81.9%), and artificial digestion (81.4%) methods were not significantly different. These results showed that the radula pressing method had a similar detection rate to traditional lung microscopy, tissue homogenization, or artificial digestion methods.
Conclusions: This study demonstrates a new method for the qualitative screening of gastropods that act as intermediate hosts of A. cantonensis (and other Angiostrongylus species), provides technical support for the control of human angiostrongyliasis, and furthers research on A. cantonensis.
(© 2024. The Author(s).)
Databáze: MEDLINE
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