Rapid Antimicrobial Susceptibility Testing Using the MicroScan System: Performance Evaluation of a 4-Hour Bacterial Cultivation From Positive Blood Cultures.

Autor: Krüger A; Wisplinghoff Laboratories, Horbeller Straße 18-20, 50858, Cologne, Germany. a.krueger1010@googlemail.com.; Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstr, 19-21, 50935, Cologne, Germany. a.krueger1010@googlemail.com., Körber-Irrgang B; Wisplinghoff Laboratories, Horbeller Straße 18-20, 50858, Cologne, Germany., Flüh G; Wisplinghoff Laboratories, Horbeller Straße 18-20, 50858, Cologne, Germany., Gielen J; Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstr, 19-21, 50935, Cologne, Germany., Scholz CJ; Wisplinghoff Laboratories, Horbeller Straße 18-20, 50858, Cologne, Germany., Wisplinghoff H; Wisplinghoff Laboratories, Horbeller Straße 18-20, 50858, Cologne, Germany.; Institute for Virology and Medical Microbiology, Witten/Herdecke University, Witten, Germany., Jazmati N; Wisplinghoff Laboratories, Horbeller Straße 18-20, 50858, Cologne, Germany.; Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Goldenfelsstr, 19-21, 50935, Cologne, Germany.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2024 Jul 09; Vol. 81 (8), pp. 261. Date of Electronic Publication: 2024 Jul 09.
DOI: 10.1007/s00284-024-03768-9
Abstrakt: A reliable and above all, rapid antimicrobial susceptibility test (AST) is required for the diganostics of blood stream infections (BSI). In this study, resistance testing using DxM MicroScan WalkAway (MicroScan) from a 4-h subculture is compared with the standard overnight culture (18-24 h). Randomly selected positive blood cultures (PBC, n = 102) with gram-negative bacteria were included in the study. PBC were sub-cultured onto appropriate agar plates and AST by MicroScan was performed after 4 h of incubation and repeated after incubation for 18-24 h as standard. In a total of 1909 drug-strain pairs, the 4-h subculture approach showed a very high essential agreement (EA) (98.6%) and categorical agreement (CA) (97.1%) compared with the standard. The incidence of minor error (mE), major error (ME), very major error (VME), and adjusted very major error (aVME) was 1.1%, 0.4%, 12.9%, and 5.3%, respectively. In summary, the use of 4-h subcultures for resistance testing with the MicroScan offers a very reliable and easy to realize time saving when testing positive blood cultures with gram-negative bacteria.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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