Performance characteristics of genus or species-specific Polymerase Chain Reaction (PCR) for the microbial diagnosis of joint infections: A systematic review and meta-analysis.

Autor: Subedi S; Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Herston, Brisbane, Australia; Sunshine Coast Hospital and Health Service, Queensland, Australia; Microbiology Laboratory, Pathology Queensland, Queensland, Australia. Electronic address: s.subedi@uqconnect.edu.au., Isler B; Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Herston, Brisbane, Australia; Infection Management Service, Princess Alexandra Hospital, Brisbane, Queensland, Australia., Ezure Y; Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Herston, Brisbane, Australia; The School of Public Health, The University of Queensland, Brisbane, Australia., Furuya-Kanamori L; Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Herston, Brisbane, Australia., Eriksson L; Herston Health Sciences Library, The University of Queensland, Brisbane, Australia., Paterson DL; Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Herston, Brisbane, Australia; ADVANCE-ID, Singapore., Harris PN; Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Herston, Brisbane, Australia; Microbiology Laboratory, Pathology Queensland, Queensland, Australia.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2024 Sep; Vol. 110 (1), pp. 116422. Date of Electronic Publication: 2024 Jul 07.
DOI: 10.1016/j.diagmicrobio.2024.116422
Abstrakt: Joint infections cause significant morbidity and mortality. Rapid diagnosis enables prompt initiation of appropriate antimicrobial therapy and surgical treatment. We conducted a systematic review and meta-analysis to evaluate the accuracy of genus- or species-specific polymerase chain reaction (PCR) in diagnosing joint infections. The literature databases were searched for articles from January 2010 to December 2022. The meta-analysis using the split component synthesis (SCS) method, included 20 studies with 2,457 adult participants. The pooled sensitivity, specificity, diagnostic odds ratio, and AUC of PCR were 49 % (95 % CI [37.9-60.2]), 95.7 % (95 % CI [91.6-97.8]), 21.32, and 0.82 respectively. Sensitivity was highest for sonicate fluid and lowest for periprosthetic tissue. The mean turnaround time to results was 4.7 hours (SD 1.1). PCR is a favourable option for diagnosing joint infections due to its rapid results, but it has low sensitivity. To enhance diagnostic yield, the test should be used in conjunction with other methods.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Patrick NA Harris reports a relationship with Gilead Sciences Inc that includes: funding grants. Patrick NA Harris reports a relationship with Pfizer Inc that includes: speaking and lecture fees. Patrick NA Harris reports a relationship with OpGen, Inc. that includes: speaking and lecture fees. Patrick NA Harris reports a relationship with Gilead Sciences Inc that includes: speaking and lecture fees. Patrick NA Harris reports a relationship with bioMérieux Inc that includes: speaking and lecture fees. Patrick NA Harris reports a relationship with OpGen, Inc. that includes: consulting or advisory. Patrick NA Harris reports a relationship with Sandoz Inc that includes: consulting or advisory. David L Paterson reports a relationship with Shionogi Inc that includes: funding grants. David L Paterson reports a relationship with Pfizer Inc that includes: funding grants. David L Paterson reports a relationship with Merck & Co Inc that includes: funding grants. David L Paterson reports a relationship with bioMérieux Inc that includes: non-financial support. David L Paterson reports a relationship with Pfizer Inc that includes: consulting or advisory. David L Paterson reports a relationship with Entos Inc that includes: consulting or advisory. David L Paterson reports a relationship with Shionogi Inc that includes: consulting or advisory. David L Paterson reports a relationship with bioMérieux Inc that includes: consulting or advisory. David L Paterson reports a relationship with Cepheid that includes: consulting or advisory. David L Paterson reports a relationship with GSK that includes: consulting or advisory. David L Paterson reports a relationship with Menarini that includes: consulting or advisory. David L Paterson reports a relationship with Aurobac Therapeutics that includes: consulting or advisory. Shradha Subedi reports a relationship with GSK that includes: funding grants. Shradha Subedi reports a relationship with bioMérieux Inc that includes: funding grants. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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