Real-Time Monitoring of Hydrolysis Reactions of Pyrophosphates with Dissolution Dynamic Nuclear Polarization.
| Autor: | Fukazawa J; Center for Quantum Information and Quantum Biology, Osaka University, Toyonaka, Osaka 560-0043, Japan., Mochizuki Y; Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan., Kanai S; Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan., Miura N; Department of Applied Biological Chemistry, Graduate School of Agriculture, Osaka Metropolitan University, Sakai, Osaka 599-8231, Japan., Negoro M; Center for Quantum Information and Quantum Biology, Osaka University, Toyonaka, Osaka 560-0043, Japan.; Institute for Quantum Life Science (iQLS), National Institutes for Quantum and Radiological Science and Technology (QST), Chiba 263-8555, Japan.; Premium Research Institute for Human Metaverse Medicine, Osaka University, Suita, Osaka 565-0871, Japan., Kagawa A; Center for Quantum Information and Quantum Biology, Osaka University, Toyonaka, Osaka 560-0043, Japan.; Premium Research Institute for Human Metaverse Medicine, Osaka University, Suita, Osaka 565-0871, Japan. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | The journal of physical chemistry letters [J Phys Chem Lett] 2024 Jul 18; Vol. 15 (28), pp. 7288-7294. Date of Electronic Publication: 2024 Jul 09. |
| DOI: | 10.1021/acs.jpclett.4c01456 |
| Abstrakt: | Dissolution dynamic nuclear polarization ( d -DNP) has enabled applications such as the real-time monitoring of chemical reactions. Such applications are mainly for 13 C and 15 N spins with long spin-lattice relaxation times in the molecules of interest. However, the only applications for phosphorus using d -DNP are pH imaging and nucleation during crystallization due to the short relaxation times. Here we show that it is possible to observe enzyme reactions using d -DNP with phosphorus. Hyperpolarized 31 P spins in pyrophosphate were obtained using bullet-DNP, which requires less dilution of highly polarized solid samples. Real-time monitoring of the hydrolysis reaction of pyrophosphate by inorganic pyrophosphatase from baker's yeast at physiological pH and was successfully achieved and the reaction rate was determined. This is an important reaction for a wide range of applications related to medicine, agriculture, and quantum life science. |
| Databáze: | MEDLINE |
| Externí odkaz: |
|