PIN2-mediated self-organizing transient auxin flow contributes to auxin maxima at the tip of Arabidopsis cotyledons.

Autor:	Pérez-Henríquez P; Institute of Integrated Genome Biology, and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA.; Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China., Nagawa S; Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China., Liu Z; Faculty of Synthetic Biology, Shenzhen University of Advanced Technology, Shenzhen, Guangdong, China.; The Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China., Pan X; Institute of Integrated Genome Biology, and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA.; Department of Biological Sciences, University of Toronto-Scarborough, Toronto, ON M1C1A4, Canada., Michniewicz M; Department of Biology, Duke University, Durham, NC, 27708, USA., Tang W; Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China., Rasmussen C; Institute of Integrated Genome Biology, and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA., Van Norman J; Institute of Integrated Genome Biology, and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA., Strader L; Department of Biology, Duke University, Durham, NC, 27708, USA., Yang Z; Institute of Integrated Genome Biology, and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA.; Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.; Faculty of Synthetic Biology, Shenzhen University of Advanced Technology, Shenzhen, Guangdong, China.; The Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Jun 28. Date of Electronic Publication: 2024 Jun 28.
DOI:	10.1101/2024.06.24.599792
Abstrakt:	Directional auxin transport and formation of auxin maxima are critical for embryogenesis, organogenesis, pattern formation, and growth coordination in plants, but the mechanisms underpinning the initiation and establishment of these auxin dynamics are not fully understood. Here we show that a self-initiating and -terminating transient auxin flow along the marginal cells (MCs) contributes to the formation of an auxin maximum at the tip of Arabidopsis cotyledon that globally coordinates the interdigitation of puzzle-shaped pavement cells in the cotyledon epidermis. Prior to the interdigitation, indole butyric acid (IBA) is converted to indole acetic acid (IAA) to induce PIN2 accumulation and polarization in the marginal cells, leading to auxin flow toward and accumulation at the cotyledon tip. When IAA levels at the cotyledon tip reaches a maximum, it activates pavement cell interdigitation as well as the accumulation of the IBA transporter TOB1 in MCs, which sequesters IBA to the vacuole and reduces IBA availability and IAA levels. The reduction of IAA levels results in PIN2 down-regulation and cessation of the auxin flow. Hence, our results elucidate a self-activating and self-terminating transient polar auxin transport system in cotyledons, contributing to the formation of localized auxin maxima that spatiotemporally coordinate pavement cell interdigitation.
Databáze:	MEDLINE
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