Colony context and size-dependent compensation mechanisms give rise to variations in nuclear growth trajectories.

Autor:	Dixon JC; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA.; These authors contributed equally to this work., Frick CL; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA.; These authors contributed equally to this work., Leveille CL; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA.; These authors contributed equally to this work., Garrison P; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA.; These authors contributed equally to this work., Lee PA; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA.; These authors contributed equally to this work., Mogre SS; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA.; These authors contributed equally to this work., Morris B; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA.; These authors contributed equally to this work., Nivedita N; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA.; These authors contributed equally to this work., Vasan R; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA.; These authors contributed equally to this work., Chen J; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA.; Present address: Leibniz-Institut fur Analytische Wissenschaften - ISAS - e.V., Dortmund, 44139, Germany., Fraser CL; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Gamlin CR; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Harris LK; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Hendershott MC; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Johnson GT; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Klein KN; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Oluoch SA; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Thirstrup DJ; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Sluzewski MF; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Wilhelm L; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Yang R; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Toloudis DM; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Viana MP; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA., Theriot JA; Department of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA., Rafelski SM; Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98109, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Jun 30. Date of Electronic Publication: 2024 Jun 30.
DOI:	10.1101/2024.06.28.601071
Abstrakt:	To investigate the fundamental question of how cellular variations arise across spatiotemporal scales in a population of identical healthy cells, we focused on nuclear growth in hiPS cell colonies as a model system. We generated a 3D timelapse dataset of thousands of nuclei over multiple days, and developed open-source tools for image and data analysis and an interactive timelapse viewer for exploring quantitative features of nuclear size and shape. We performed a data-driven analysis of nuclear growth variations across timescales. We found that individual nuclear volume growth trajectories arise from short timescale variations attributable to their spatiotemporal context within the colony. We identified a strikingly time-invariant volume compensation relationship between nuclear growth duration and starting volume across the population. Notably, we discovered that inheritance plays a crucial role in determining these two key nuclear growth features while other growth features are determined by their spatiotemporal context and are not inherited. Competing Interests: Declaration of interests The authors declare no competing interests.
Databáze:	MEDLINE
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