Fitness costs of Tn1546-type transposons harboring the vanA operon by plasmid type and structural diversity in Enterococcus faecium.

Autor: Kim D; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Gangnam Severance Hospita, l, Yonsei University College of Medicine, 211 Eonju-Ro, Gangnam-Gu, Seoul, 06273, South Korea., Kang DY; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Gangnam Severance Hospita, l, Yonsei University College of Medicine, 211 Eonju-Ro, Gangnam-Gu, Seoul, 06273, South Korea., Choi MH; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Gangnam Severance Hospita, l, Yonsei University College of Medicine, 211 Eonju-Ro, Gangnam-Gu, Seoul, 06273, South Korea., Hong JS; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Gangnam Severance Hospita, l, Yonsei University College of Medicine, 211 Eonju-Ro, Gangnam-Gu, Seoul, 06273, South Korea.; Department of Companion Animal Health and Science, Silla University, Busan, South Korea., Kim HS; Department of Laboratory Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, South Korea., Kim YR; Department of Laboratory Medicine, Jeju National University College of Medicine, Jeju, South Korea., Kim YA; Department of Laboratory Medicine, National Health Insurance Service, Ilsan Hospital, Goyang, South Korea., Uh Y; Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, South Korea., Shin KS; Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, South Korea., Shin JH; Department of Laboratory Medicine and Paik Institute for Clinical Research, Inje University College of Medicine, Busan, South Korea., Kim SH; Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea., Shin JH; Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, South Korea., Jeong SH; Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Gangnam Severance Hospita, l, Yonsei University College of Medicine, 211 Eonju-Ro, Gangnam-Gu, Seoul, 06273, South Korea. kscpjsh@yuhs.ac.
Jazyk: angličtina
Zdroj: Annals of clinical microbiology and antimicrobials [Ann Clin Microbiol Antimicrob] 2024 Jul 08; Vol. 23 (1), pp. 62. Date of Electronic Publication: 2024 Jul 08.
DOI: 10.1186/s12941-024-00722-2
Abstrakt: Background: This study analyzed the genetic traits and fitness costs of vancomycin-resistant Enterococcus faecium (VREfm) blood isolates carrying Tn1546-type transposons harboring the vanA operon.
Methods: All E. faecium blood isolates were collected from eight general hospitals in South Korea during one-year study period. Antimicrobial susceptibility testing and vanA and vanB PCR were performed. Growth rates of E. faecium isolates were determined. The vanA-positive isolates were subjected to whole genome sequencing and conjugation experiments.
Results: Among 308 E. faecium isolates, 132 (42.9%) were positive for vanA. All Tn1546-type transposons harboring the vanA operon located on the plasmids, but on the chromosome in seven isolates. The plasmids harboring the vanA operon were grouped into four types; two types of circular, nonconjugative plasmids (Type A, n = 50; Type B, n = 46), and two types of putative linear, conjugative plasmids (Type C, n = 16; Type D, n = 5). Growth rates of vanA-positive E. faecium isolates were significantly lower than those of vanA-negative isolates (P < 0.001), and reduction in growth rate under vancomycin pressure was significantly larger in isolates harboring putative linear plasmids than in those harboring circular plasmids (P = 0.020).
Conclusions: The possession of vanA operon was costly to bacterial hosts in antimicrobial-free environment, which provide evidence for the importance of reducing vancomycin pressure for prevention of VREfm dissemination. Fitness burden to bacterial hosts was varied by type and size of the vanA operon-harboring plasmid.
(© 2024. The Author(s).)
Databáze: MEDLINE
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