A single-cell atlas of chromatin accessibility in mouse organogenesis.

Autor: Sun K; School of Medicine, Tsinghua University, Beijing, China.; Peking-Tsinghua-NIBS Joint Graduate Program, Tsinghua University, Beijing, China.; Tsinghua-Peking Center for Life Sciences, Beijing, China., Liu X; Tsinghua-Peking Center for Life Sciences, Beijing, China.; School of Life Sciences, Tsinghua University, Beijing, China., Lan X; School of Medicine, Tsinghua University, Beijing, China. xlan@mail.tsinghua.edu.cn.; Peking-Tsinghua-NIBS Joint Graduate Program, Tsinghua University, Beijing, China. xlan@mail.tsinghua.edu.cn.; Tsinghua-Peking Center for Life Sciences, Beijing, China. xlan@mail.tsinghua.edu.cn.; MOE Key Laboratory of Bioinformatics, Tsinghua University, Beijing, China. xlan@mail.tsinghua.edu.cn.
Jazyk: angličtina
Zdroj: Nature cell biology [Nat Cell Biol] 2024 Jul; Vol. 26 (7), pp. 1200-1211. Date of Electronic Publication: 2024 Jul 08.
DOI: 10.1038/s41556-024-01435-6
Abstrakt: Organogenesis is a highly complex and precisely regulated process. Here we profiled the chromatin accessibility in >350,000 cells derived from 13 mouse embryos at four developmental stages from embryonic day (E) 10.5 to E13.5 by SPATAC-seq in a single experiment. The resulting atlas revealed the status of 830,873 candidate cis-regulatory elements in 43 major cell types. By integrating the chromatin accessibility atlas with the previous transcriptomic dataset, we characterized cis-regulatory sequences and transcription factors associated with cell fate commitment, such as Nr5a2 in the development of gastrointestinal tract, which was preliminarily supported by the in vivo experiment in zebrafish. Finally, we integrated this atlas with the previous single-cell chromatin accessibility dataset from 13 adult mouse tissues to delineate the developmental stage-specific gene regulatory programmes within and across different cell types and identify potential molecular switches throughout lineage development. This comprehensive dataset provides a foundation for exploring transcriptional regulation in organogenesis.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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