Exploring rose absolute and phenylethyl alcohol as novel quorum sensing inhibitors in Pseudomonas aeruginosa and Chromobacterium violaceum.
| Autor: | Çevikbaş H; Faculty of Engineering and Natural Sciences, Biology Department, Süleyman Demirel University, Isparta, 32260, Turkey., Ulusoy S; Faculty of Engineering and Natural Sciences, Biology Department, Süleyman Demirel University, Isparta, 32260, Turkey. seyhanulusoy@sdu.edu.tr., Kaya Kinaytürk N; Faculty of Arts and Science, Nanoscience and Nanotechnology Department, Mehmet Akif Ersoy University, Burdur, 15100, Turkey. |
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| Jazyk: | angličtina |
| Zdroj: | Scientific reports [Sci Rep] 2024 Jul 08; Vol. 14 (1), pp. 15666. Date of Electronic Publication: 2024 Jul 08. |
| DOI: | 10.1038/s41598-024-66888-z |
| Abstrakt: | Inter-cellular signaling, referred to as quorum sensing (QS), regulates the production of virulence factors in numerous gram-negative bacteria, such as the human pathogens Pseudomonas aeruginosa and Chromobacterium violaceum. QS inhibition may provide an opportunity for the treatment of bacterial infections. This represents the initial study to examine the antibiofilm and antivirulence capabilities of rose absolute and its primary component, phenylethyl alcohol. QS inhibition was assessed by examining extracellular exopolysaccharide synthesis, biofilm development, and swarming motility in P. aeruginosa PAO1, along with violacein production in C. violaceum ATCC 12472. Molecular docking analysis was conducted to explore the mechanism by which PEA inhibits QS. Our results indicate that rose absolute and PEA caused decrease in EPS production (60.5-33.5%), swarming motility (94.7-64.5%), and biofilm formation (98.53-55.5%) in the human pathogen P. aeruginosa PAO1. Violacein production decreased by 98.1% and 62.5% with an absolute (0.5 v/v %) and PEA (2 mM). Moreover, the molecular docking analysis revealed a promising competitive interaction between PEA and AHLs. Consequently, this study offers valuable insights into the potential of rose absolute and PEA as inhibitors of QS in P. aeruginosa and C. violaceum. (© 2024. The Author(s).) |
| Databáze: | MEDLINE |
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