N-terminal truncated trehalose-6-phosphate synthase 1 gene (△NIbTPS1) enhances the tolerance of sweet potato to abiotic stress.

Autor: Zou X; College of Life Sciences, Shanxi Agricultural University, Taigu, 030801, China., Wang S; College of Life Sciences, Shanxi Agricultural University, Taigu, 030801, China., Cheng Q; College of Life Sciences, Shanxi Agricultural University, Taigu, 030801, China., Yu H; College of Life Sciences, Shanxi Agricultural University, Taigu, 030801, China., Yang Z; College of Life Sciences, Shanxi Agricultural University, Taigu, 030801, China., Wang Y; College of Horticulture, Shanxi Agricultural University, Taigu, 030801, China., Yang Y; College of Basic Sciences, Shanxi Agricultural University, Taigu, 030801, China., Liang X; College of Life Sciences, Shanxi Agricultural University, Taigu, 030801, China., Yang D; Key Laboratory of Biology and Genetic Improvement of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Xuzhou, Jiangsu, 221131, China., Kim HS; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Daejeon, 34141, South Korea., Jia X; College of Life Sciences, Shanxi Agricultural University, Taigu, 030801, China., Li L; College of Horticulture, Shanxi Agricultural University, Taigu, 030801, China., Kwak SS; Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Daejeon, 34141, South Korea. Electronic address: sskwak@kribb.re.kr., Wang W; College of Life Sciences, Shanxi Agricultural University, Taigu, 030801, China. Electronic address: sxndwwb@126.com.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Sep; Vol. 214, pp. 108917. Date of Electronic Publication: 2024 Jul 06.
DOI: 10.1016/j.plaphy.2024.108917
Abstrakt: Sweet potato [Ipomoea batatas (L.) Lam], the crop with the seventh highest annual production globally, is susceptible to various adverse environmental influences, and the study of stress-resistant genes is important for improving its tolerance to abiotic stress. The enzyme trehalose-6-phosphate synthase (TPS) is indispensable in the one pathway for synthesizing trehalose in plants. TPS is known to participate in stress response in plants, but information on TPS in sweet potato is limited. This study produced the N-terminal truncated IbTPS1 gene (△NIbTPS1) overexpression lines of Arabidopsis thaliana and sweet potato. Following salt and mannitol-induced drought treatment, the germination rate, root elongation, and fresh weight of the transgenic A. thaliana were significantly higher than that in the wild type. Overexpression of △NIbTPS1 elevated the photosynthetic efficiency (Fv/Fm) and the activity of superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase in sweet potato during drought and salt treatments, while reducing malondialdehyde and O 2 ∙- contents, although expression of the trehalose-6-phosphate phosphatase gene IbTPP and trehalose concentrations were not affected. Thus, overexpressing the △NIbTPS1 gene can improve the stress tolerance of sweet potato to drought and salt by enhancing the photosynthetic efficiency and antioxidative enzyme system. These results will contribute to understand the functions of the △NIbTPS1 gene and trehalose in the response mechanism of higher plants to abiotic stress.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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