An integrated magnetic separation enzyme-linked colorimetric sensing platform for field detection of Escherichia coli O157: H7 in food.

Autor: Wang C; Institute of Biomedical Precision Testing and Instrumentation, College of Biomedical Engineering, Taiyuan University of Technology, Jinzhong, 030600, Shanxi, China., Deng R; Institute of Biomedical Precision Testing and Instrumentation, College of Biomedical Engineering, Taiyuan University of Technology, Jinzhong, 030600, Shanxi, China. dengrong@tyut.edu.cn., Li H; Institute of Biomedical Precision Testing and Instrumentation, College of Biomedical Engineering, Taiyuan University of Technology, Jinzhong, 030600, Shanxi, China., Liu Z; Analysis & Testing Center, Jilin Institute of Chemical Technology, Jilin, 132000, Jilin, China., Niu X; Taiyuan Liuweizhai Industrial Co., Ltd, Taiyuan, 030024, Shanxi, China., Li X; Institute of Biomedical Precision Testing and Instrumentation, College of Biomedical Engineering, Taiyuan University of Technology, Jinzhong, 030600, Shanxi, China. lixiaochun@tyut.edu.cn.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Jul 08; Vol. 191 (8), pp. 454. Date of Electronic Publication: 2024 Jul 08.
DOI: 10.1007/s00604-024-06497-9
Abstrakt: An intelligent colorimetric sensing platform integrated with in situ immunomagnetic separation function was developed for ultrasensitive detection of Escherichia coli O157: H7 (E. coli O157: H7) in food. Captured antibody modified magnetic nanoparticles (cMNPs) and detection antibody/horseradish peroxidase (HRP) co-functionalized AuNPs (dHAuNPs) were firstly synthesized for targeted enrichment and colorimetric assay of E. coli O157: H7, in which remarkable signal amplification was realized by loading large amounts of HRP on the surface of AuNPs. Coupling with the optical collimation attachments and embedded magnetic separation module, a highly integrated optical device was constructed, by which in situ magnetic separation and high-quality imaging of 96-well microplates containing E. coli O157: H7 was achieved with a smartphone. The concentration of E. coli O157: H7 could be achieved in one-step by performing digital image colorimetric analysis of the obtained image with a custom-designed app. This biosensor possesses high sensitivity (1.63 CFU/mL), short detecting time (3 h), and good anti-interference performance even in real-sample testing. Overall, the developed method is expected to be a novel field detection platform for foodborne pathogens in water and food as well as for the diagnosis of infections due to its portability, ease of operation, and high feasibility.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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