Improvement of a low-cost protocol for a simultaneous comparative evaluation of hydrolytic activity between sessile and planktonic cells: Candida albicans as a study model.

Autor: Kendil W; Antifungal Antibiotic: Physico-Chemical Synthesis and Biological Activity Laboratory (LAPSAB), Biology Department, University of Tlemcen, Tlemcen, Algeria., Dergal F; Scientific and Technical Research Center in Physico-Chemical Analysis (CRAPC), Tipaza, Algeria.; Laboratory of Catalysis and Synthesis in Organic Chemistry (LCSCO), Faculty of Sciences, University of Tlemcen, Tlemcen, Algeria., Tefiani I; Antifungal Antibiotic: Physico-Chemical Synthesis and Biological Activity Laboratory (LAPSAB), Biology Department, University of Tlemcen, Tlemcen, Algeria., Mahdad YM; Laboratory for Sustainable Management of Natural Resources in Arid and Semi-Arid Areas, University Center of Naâma, Naâma, Algeria.; Laboratory of Applied Genetic in Agriculture, Ecology and Public Health, University of Tlemcen, Tlemcen, Algeria., Benladghem Z; Antifungal Antibiotic: Physico-Chemical Synthesis and Biological Activity Laboratory (LAPSAB), Biology Department, University of Tlemcen, Tlemcen, Algeria.; Department of Biology, University of Tamanrasset, Tamanrasset, Algeria., Ziani-Cherif C; Laboratory of Catalysis and Synthesis in Organic Chemistry (LCSCO), Faculty of Sciences, University of Tlemcen, Tlemcen, Algeria., Seddiki SML; Antifungal Antibiotic: Physico-Chemical Synthesis and Biological Activity Laboratory (LAPSAB), Biology Department, University of Tlemcen, Tlemcen, Algeria.; Laboratory for Sustainable Management of Natural Resources in Arid and Semi-Arid Areas, University Center of Naâma, Naâma, Algeria.
Jazyk: angličtina
Zdroj: Biofouling [Biofouling] 2024 Aug; Vol. 40 (7), pp. 431-445. Date of Electronic Publication: 2024 Jul 07.
DOI: 10.1080/08927014.2024.2376637
Abstrakt: Candida albicans is often implicated in nosocomial infections with fatal consequences. Its virulence is contributed to hydrolytic enzymes and biofilm formation. Previous research focused on studying these virulence factors individually. Therefore, this study aimed to investigate the impact of biofilm formation on the hydrolytic activity using an adapted low-cost method. Eleven strains of C. albicans were used. The biofilms were formed on pre-treated silicone discs using 24-well plates and then deposited on the appropriate agar to test each enzyme, while the planktonic cells were conventionally seeded. Biofilms were analysed using Raman spectroscopy, fluorescent and scanning electron microscopy. The adapted method provided an evaluation of hydrolytic enzymes activity in C. albicans biofilm and showed that sessile cells had a higher phospholipase and proteinase activities compared with planktonic cells. These findings were supported by spectroscopic and microscopic analyses, which provided valuable insights into the virulence mechanisms of C. albicans during biofilm formation.
Databáze: MEDLINE