A gibberellin-assisted study of the transcriptional and hormonal changes occurring at floral transition in peach buds (Prunus persica L. Batsch).

Autor: Girardi F; Department of Agronomy, Food, Natural resources, Animals and Environment - DAFNAE, University of Padova, Agripolis, Viale dell'università 16, Legnaro, PD, 35020, Italy., Canton M; Department of Agronomy, Food, Natural resources, Animals and Environment - DAFNAE, University of Padova, Agripolis, Viale dell'università 16, Legnaro, PD, 35020, Italy., Populin F; Department of Agronomy, Food, Natural resources, Animals and Environment - DAFNAE, University of Padova, Agripolis, Viale dell'università 16, Legnaro, PD, 35020, Italy.; Berry Genetics and Breeding Unit - Research and Innovation Centre (CRI), Fondazione Edmund Mach, San Michele all'Adige, Trento, 38098, Italy., Tijero V; Department of Agronomy, Food, Natural resources, Animals and Environment - DAFNAE, University of Padova, Agripolis, Viale dell'università 16, Legnaro, PD, 35020, Italy.; Fruit Production Programme, Institute of Agrifood Research and Technology (IRTA), Parc Agrobiotech Lleida, Parc de Gardeny, Edifici Fruitcentre, Lleida, 25003, Spain., Bettio G; Department of Agronomy, Food, Natural resources, Animals and Environment - DAFNAE, University of Padova, Agripolis, Viale dell'università 16, Legnaro, PD, 35020, Italy., Munné-Bosch S; Department of Evolutionary Biology, Ecology and Environmental Sciences, University of Barcelona, Diagonal 643, Barcelona, 08017, Spain., Rasori A; Department of Agronomy, Food, Natural resources, Animals and Environment - DAFNAE, University of Padova, Agripolis, Viale dell'università 16, Legnaro, PD, 35020, Italy., Cardillo V; Department of Agronomy, Food, Natural resources, Animals and Environment - DAFNAE, University of Padova, Agripolis, Viale dell'università 16, Legnaro, PD, 35020, Italy., Costa G; Department of Agricultural and Food Sciences - DISTAL, University of Bologna, Bologna, 40126, Italy., Botton A; Department of Agronomy, Food, Natural resources, Animals and Environment - DAFNAE, University of Padova, Agripolis, Viale dell'università 16, Legnaro, PD, 35020, Italy. alessandro.botton@unipd.it.
Jazyk: angličtina
Zdroj: BMC plant biology [BMC Plant Biol] 2024 Jul 08; Vol. 24 (1), pp. 643. Date of Electronic Publication: 2024 Jul 08.
DOI: 10.1186/s12870-024-05360-6
Abstrakt: Background: Flower load in peach is an important determinant of final fruit quality and is subjected to cost-effective agronomical practices, such as the thinning, to finely balance the sink-source relationships within the tree and drive the optimal amount of assimilates to the fruits. Floral transition in peach buds occurs as a result of the integration of specific environmental signals, such as light and temperature, into the endogenous pathways that induce the meristem to pass from vegetative to reproductive growth. The cross talk and integration of the different players, such as the genes and the hormones, are still partially unknown. In the present research, transcriptomics and hormone profiling were applied on bud samples at different developmental stages. A gibberellin treatment was used as a tool to identify the different phases of floral transition and characterize the bud sensitivity to gibberellins in terms of inhibition of floral transition.
Results: Treatments with gibberellins showed different efficacies and pointed out a timeframe of maximum inhibition of floral transition in peach buds. Contextually, APETALA1 gene expression was shown to be a reliable marker of gibberellin efficacy in controlling this process. RNA-Seq transcriptomic analyses allowed to identify specific genes dealing with ROS, cell cycle, T6P, floral induction control and other processes, which are correlated with the bud sensitivity to gibberellins and possibly involved in bud development during its transition to the reproductive stage. Transcriptomic data integrated with the quantification of the main bioactive hormones in the bud allowed to identify the main hormonal regulators of floral transition in peach, with a pivotal role played by endogenous gibberellins and cytokinins.
Conclusions: The peach bud undergoes different levels of receptivity to gibberellin inhibition. The stage with maximum responsiveness corresponded to a transcriptional and hormonal crossroad, involving both flowering inhibitors and inductors. Endogenous gibberellin levels increased only at the latest developmental stage, when floral transition was already partially achieved, and the bud was less sensitive to exogenous treatments. A physiological model summarizes the main findings and suggests new research ideas to improve our knowledge about floral transition in peach.
(© 2024. The Author(s).)
Databáze: MEDLINE
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