Gut colonization by extended-spectrum β-lactamase-producing Escherichia coli in dairy herd in Brazil: successful dissemination of a One Health clone.

Autor: Sartori L; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil., Sellera FP; Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.; School of Veterinary Medicine, Metropolitan University of Santos, Santos, Brazil.; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil., Silva-Pereira TT; Departmentof Microbiology, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil., Fuga B; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil.; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil.; Departmentof Microbiology, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil., Fuentes-Castillo D; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil.; Departamento de Patología y Medicina Preventiva, Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán, Chile., Dropa M; MicroRes Laboratory, School of Public Health, University of São Paulo, São Paulo, Brazil., Moura Q; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil.; Federal Institute of Espírito Santo, Vila Velha, Brazil., Fernandes MR; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil., Rodrigues L; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil.; Departmentof Microbiology, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil., Esposito F; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil.; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil., Sano E; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil.; Departmentof Microbiology, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil., Aleman MAR; Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Gregory L; Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Lincopan N; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil. lincopan@usp.br.; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil. lincopan@usp.br.; Departmentof Microbiology, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil. lincopan@usp.br.
Jazyk: angličtina
Zdroj: Veterinary research communications [Vet Res Commun] 2024 Oct; Vol. 48 (5), pp. 3355-3363. Date of Electronic Publication: 2024 Jul 08.
DOI: 10.1007/s11259-024-10456-3
Abstrakt: The overuse of antimicrobials in livestock has contributed to the emergence and selection of clinically relevant multidrug-resistant bacteria. In Brazil, there is no conclusive information on the occurrence of Escherichia coli producing extended-spectrum β-lactamase (ESβL) in cattle breeding, which is an important sector of agribusiness in this country. Herein, we investigated the presence of ESβL-positive E. coli strains in dairy cattle from a commercial farm with routine practice of therapeutic cephalosporins. Ninety-five rectal swab samples were collected from healthy dairy calves and cows under treatment with ceftiofur. Samples were screened for the presence of ESβL producers, and positive isolates were identified by MALDI-TOF, with subsequent screening for genes encoding ESβL variants by PCR and sequencing. The presence of ESβL (CTX-M-15)-producing E. coli was confirmed in calves, and lactating and dry cows. Most ESβL strains with genetic homologies ≥ 90% were grouped into two major PFGE clusters, confirming the suscessful expansion of clonally related lineages in animals from different lactating cycles, on the same property. Four representatives CTX-M-15-positive E. coli strains had their genomes sequenced, belonging to the clonal complex (CC) 23 and sequence type (ST) 90. A phylogeographical landscape of ST90 was performed revealing a global One Health linkage. Our results highlight the intestinal microbiota of dairy cattle as a hotspot for the spread of critical priority ESβL-producing E. coli and demonstrate that ST90 is an international clone genomically adapted to human and animal hosts, which deserve additional investigation to determine its zoonotic potential and impact in food chain.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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