Mechanism underlying the role of integrin α3β1 in adhesive dysfunction between thyroid cells induced by diesel engine exhaust particles.

Autor: Guo X; School of Public Health, Qingdao University, Qingdao, China., Tang S; Chengyang City Centre for Disease Control and Prevention, Qingdao, China., Li Y; School of Public Health, Qingdao University, Qingdao, China., Mu C; Department of Respiratory and Critical Care Medicine, Qingdao Municipal Hospital, Qingdao, China., Zhang H; School of Public Health, Qingdao University, Qingdao, China., Jiang Q; School of Public Health, Qingdao University, Qingdao, China., Jiang M; School of Public Health, Qingdao University, Qingdao, China., Han W; Department of Respiratory and Critical Care Medicine, Qingdao Municipal Hospital, Qingdao, China., Zheng Y; School of Public Health, Qingdao University, Qingdao, China. Electronic address: yxzheng@qdu.edu.cn., Piao J; School of Public Health, Qingdao University, Qingdao, China. Electronic address: jinmeipark@163.com.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Oct 15; Vol. 947, pp. 174535. Date of Electronic Publication: 2024 Jul 05.
DOI: 10.1016/j.scitotenv.2024.174535
Abstrakt: The role and mechanisms of DEP exposure on thyroid injury are not yet clear. This study explores thyroid damage induced by in vivo DEP exposure using a mouse model. This study has observed alterations in thyroid follicular architecture, including rupture, colloid overflow, and the formation of voids. Additionally, there was a significant decrease in the expression levels of proteins involved in thyroid hormone synthesis, such as thyroid peroxidase and thyroglobulin, their trend of change is consistent with the damage to the thyroid structure. Serum levels of triiodothyronine and tetraiodothyronine were raise. However, the decrease in TSH expression suggests that the function of the HPT axis is unaffected. To delve deeper into the intrinsic mechanisms of thyroid injury, we performed KEGG pathway enrichment analysis, which revealed notable alterations in the cell adhesion signaling pathway. Our immunofluorescence results show that DEP exposure impairs thyroid adhesion, and integrin α3β1 plays an important role. CD151 binds to α3β1, promoting multimolecular complex formation and activating adhesion-dependent small GTPases. Our in vitro model has confirmed the pivotal role of integrin α3β1 in thyroid cell adhesion, which may be mediated by the CD151/α3β1/Rac1 pathway. In summary, exposure to DEP disrupts the structure and function of the thyroid, a process that likely involves the regulation of cell adhesion through the CD151/α3β1/Rac1 pathway, leading to glandular damage.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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