Effects of producing high levels of hyperthermophile-specific C 25 ,C 25 -archaeal membrane lipids in Escherichia coli.

Autor: Yoshida R; Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 460-8601, Japan., Motoyama K; Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 460-8601, Japan., Ito T; Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 460-8601, Japan., Hemmi H; Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 460-8601, Japan. Electronic address: hhemmi@agr.nagoya-u.ac.jp.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Oct 15; Vol. 729, pp. 150349. Date of Electronic Publication: 2024 Jul 04.
DOI: 10.1016/j.bbrc.2024.150349
Abstrakt: A hyperthermophilic archaeon, Aeropyrum pernix, synthesizes C 25 ,C 25 -archaeal membrane lipids, or extended archaeal membrane lipids, which contain two C 25 isoprenoid chains that are linked to glycerol-1-phosphate via ether bonds and are longer than the usual C 20 ,C 20 -archaeal membrane lipids. The C 25 ,C 25 -archaeal membrane lipids are believed to allow the archaeon to survive under harsh conditions, because they are able to form lipid membranes that are impermeable at temperatures approaching the boiling point. The effect that C 25 ,C 25 -archaeal membrane lipids exert on living cells, however, remains unproven along with an explanation for why the hyperthermophilic archaeon synthesizes these specific lipids instead of the more common C 20 ,C 20 -archaeal lipids or double-headed tetraether lipids. To shed light on the effects that these hyperthermophile-specific membrane lipids exert on living cells, we have constructed an E. coli strain that produces C 25 ,C 25 -archaeal membrane lipids. However, a resultant low level of productivity would not allow us to assess the effects of their production in E. coli cells. Herein, we report an enhancement of the productivity of C 25 ,C 25 -archaeal membrane lipids in engineered E. coli strains via the introduction of metabolic pathways such as an artificial isoprenol utilization pathway where the precursors of isoprenoids are synthesized via a two-step phosphorylation of prenol and isoprenol supplemented to a growth medium. In the strain with the highest titer, a major component of C 25 ,C 25 -archaeal membrane lipids reached ∼11 % of total lipids of E. coli. It is noteworthy that the high production of the extended archaeal lipids did not significantly affect the growth of the bacterial cells. The permeability of the cell membrane of the strain became slightly lower in the presence of the exogenous membrane lipids with longer hydrocarbon chains, which demonstrated the possibility to enhance bacterial cell membranes by the hyperthermophile-specific lipids, along with the surprising robustness of the E. coli cell membrane.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Hisashi Hemmi reports financial support was provided by Japan Society for the Promotion of Science. Hisashi Hemmi reports financial support was provided by Institute for Fermentation Osaka. Hisashi Hemmi reports financial support was provided by Noda Institute for Scientific Research. Hisashi Hemmi reports financial support was provided by Nagase Scientific Technology Foundation. Ryo Yoshida reports financial support was provided by Japan Society for the Promotion of Science. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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