A novel murine model mimicking male genital Neisseria species infection using Neisseria musculi†.

Autor:	Bryan ER; Faculty of Health, School of Biomedical Science, Centre for Immunology and Infection Control, Queensland University of Technology, Brisbane, Queensland 4006, Australia.; Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Brisbane, Queensland 4029, Australia., McRae J; Faculty of Health, School of Biomedical Science, Centre for Immunology and Infection Control, Queensland University of Technology, Brisbane, Queensland 4006, Australia., Kumar V; Justus-Liebig-University Giessen, Institute for Anatomy and Cell Biology, 35385 Giessen, Germany., Trim LK; Faculty of Health, School of Biomedical Science, Centre for Immunology and Infection Control, Queensland University of Technology, Brisbane, Queensland 4006, Australia., Maidment TI; Faculty of Health, School of Biomedical Science, Centre for Immunology and Infection Control, Queensland University of Technology, Brisbane, Queensland 4006, Australia.; Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Brisbane, Queensland 4029, Australia., Tickner JAD; Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Brisbane, Queensland 4029, Australia., Sweeney EL; Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Brisbane, Queensland 4029, Australia., Williams ED; Faculty of Health, School of Biomedical Science at Translational Research Institute, Centre for Genomics and Personalised Health, Queensland University of Technology, Brisbane, Queensland 4102, Australia., Whiley DM; Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Brisbane, Queensland 4029, Australia., Beagley KW; Faculty of Health, School of Biomedical Science, Centre for Immunology and Infection Control, Queensland University of Technology, Brisbane, Queensland 4006, Australia.
Jazyk:	angličtina
Zdroj:	Biology of reproduction [Biol Reprod] 2024 Sep 14; Vol. 111 (3), pp. 613-624.
DOI:	10.1093/biolre/ioae100
Abstrakt:	With ~78 million cases yearly, the sexually transmitted bacterium Neisseria gonorrhoeae is an urgent threat to global public health due to continued emergence of antimicrobial resistance. In the male reproductive tract, untreated infections may cause permanent damage, poor sperm quality, and subsequently subfertility. Currently, few animal models exist for N. gonorrhoeae infection, which has strict human tropism, and available models have limited translatability to human disease. The absence of appropriate models inhibits the development of vital new diagnostics and treatments. However, the discovery of Neisseria musculi, a mouse oral cavity bacterium, offers much promise. This bacterium has already been used to develop an oral Neisseria infection model, but the feasibility of establishing urogenital gonococcal models is unexplored. We inoculated mice via the intrapenile route with N. musculi. We assessed bacterial burden throughout the male reproductive tract, the systemic and tissue-specific immune response 2-weeks postinfection, and the effect of infection on sperm health. Neisseria musculi was found in penis (2/5) and vas deferens (3/5) tissues. Infection altered immune cell counts: CD19+ (spleen, lymph node, penis), F4/80+ (spleen, lymph node, epididymus), and Gr1+ (penis) compared with noninfected mice. This culminated in sperm from infected mice having poor viability, motility, and morphology. We hypothesize that in the absence of testis infection, infection and inflammation in other reproductive is sufficient to damage sperm quality. Many results herein are consistent with outcomes of gonorrhoea infection, indicating the potential of this model as a tool for enhancing the understanding of Neisseria infections of the human male reproductive tract. (© The Author(s) 2024. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu