A live cell imaging-based assay for tracking particle uptake by clathrin-mediated endocytosis.
| Autor: | Ashby G; Department of Biomedical Engineering, The University of Texas at Austin., Keng KE; Department of Biomedical Engineering, The University of Texas at Austin., Hayden CC; Department of Biomedical Engineering, The University of Texas at Austin., Stachowiak JC; Department of Biomedical Engineering, The University of Texas at Austin; Department of Chemical Engineering, The University of Texas at Austin. Electronic address: jcstach@austin.utexas.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in enzymology [Methods Enzymol] 2024; Vol. 700, pp. 413-454. Date of Electronic Publication: 2024 Mar 22. |
| DOI: | 10.1016/bs.mie.2024.02.010 |
| Abstrakt: | A popular strategy for therapeutic delivery to cells and tissues is to encapsulate therapeutics inside particles that cells internalize via endocytosis. The efficacy of particle uptake by endocytosis is often studied in bulk using flow cytometry and Western blot analysis and confirmed using confocal microscopy. However, these techniques do not reveal the detailed dynamics of particle internalization and how the inherent heterogeneity of many types of particles may impact their endocytic uptake. Toward addressing these gaps, here we present a live-cell imaging-based method that utilizes total internal reflection fluorescence microscopy to track the uptake of a large ensemble of individual particles in parallel, as they interact with the cellular endocytic machinery. To analyze the resulting data, we employ an open-source tracking algorithm in combination with custom data filters. This analysis reveals the dynamic interactions between particles and endocytic structures, which determine the probability of particle uptake. In particular, our approach can be used to examine how variations in the physical properties of particles (size, targeting, rigidity), as well as heterogeneity within the particle population, impact endocytic uptake. These data impact the design of particles toward more selective and efficient delivery of therapeutics to cells. (Copyright © 2024. Published by Elsevier Inc.) |
| Databáze: | MEDLINE |
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