Changes in the gut microbiome of patients with esophageal cancer: A systematic review and meta-analysis based on 16S gene sequencing technology.

Autor: Deng J; The Affiliated Hospital, Southwest Medical University, Luzhou 611630, China; Department of General Medicine, General Hospital of PLA Western Theater Command, Chengdu 610083, China., Huang Y; Department of Nursing, Nursing School, Chengdu Medical College, Chengdu 610083, China., Yu K; Department of General Medicine, General Hospital of PLA Western Theater Command, Chengdu 610083, China., Luo H; Department of Oncology, General Hospital of PLA Western Theater Command, Chengdu 610083, China., Zhou D; Department of Oncology, General Hospital of PLA Western Theater Command, Chengdu 610083, China. Electronic address: daijunzhou@vip.qq.com., Li D; The Affiliated Hospital, Southwest Medical University, Luzhou 611630, China; Department of Oncology, General Hospital of PLA Western Theater Command, Chengdu 610083, China. Electronic address: 13438078785@163.com.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2024 Aug; Vol. 193, pp. 106784. Date of Electronic Publication: 2024 Jul 04.
DOI: 10.1016/j.micpath.2024.106784
Abstrakt: Background: Esophageal cancer (EC) possesses a high degree of malignancy and exhibits poor therapeutic outcomes and prognosis. However, its pathogenesis remains unclear. With the development of macrogene sequencing technology, changes in the intestinal flora have been found to be highly related to the development of EC, although discrepancies and controversies remain in this research area.
Materials and Methods: We comprehensively searched the PubMed, EMBASE, and Cochrane's Central Controlled Trials Register and the Scientific Network's database search projects based on systematically reviewed preferred reporting projects and meta-analyses. We used Engauge Digitizer for data extraction and Stata 15.1 for data analysis. In addition, we used the Newcastle-Ottawa Scale for grade grading and forest and funnel plots, sensitivity, and Egger and Beggar tests to evaluate the risk of bias.
Results: This study included 10 studies that assessed stool, tumor, and nontumor esophageal mucosa (gastroscopy and surgical resection) samples from 527 individuals, including 273 patients with EC and 254 healthy control group. We observed remarkable differences in microbial diversity in EC patients compared to healthy controls. The Chao1 index (46.01 vs. 42.67) was significantly increased in EC patients, whereas the Shannon index (14.90 vs. 19.05), ACE (39.24 vs. 58.47), and OTUs(28.93 vs. 70.10) were significantly lower. At the phylum level, the abundance of Bacteroidetes (37.89 vs. 32.77) increased significantly, whereas that of Firmicutes (37.63 vs. 38.72) decreased significantly; the abundance of Clostridium and Verruciformis increased, while that of Actinobacteria and Proteobacteria decreased to varying degrees. The abundance of Bacteroides (8.60 vs. 15.10) and Streptococcaceae (15.08 vs. 27.05) significantly reduced in EC.
Conclusions: According to our meta-analysis, in patients with EC, the Chao1 index increased, whereas the Shannon and the OTUs decreased. At the phylum level, the abundance of Firmicutes decreased significantly, whereas that of Bacteroidetes and Proteobacteria increased significantly. At the genus/family level, the abundance of Bacteroidaceae, Prevotellaceae and Streptococcaceae decreased significantly, whereas that of Veillonellaceae increased. This meta-analysis identified changes in gut microbiota in patients with EC; however, its conclusions were inconsistent.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dong LI reports financial support was provided by National Natural Science Foundation of China. Dong LI reports a relationship with National Natural Science Foundation of China that includes: funding grants. Hong LUO has patent #82102920 issued to Dong LI、Hong LUO. No conflict of interest If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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