Biological potential, chemical profiling, and molecular docking study of Morus alba L. extracts.

Autor: Mahmutović-Dizdarević I; University of Sarajevo-Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and Herzegovina. Electronic address: irma.m@pmf.unsa.ba., Mesic A; University of Sarajevo-Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and Herzegovina., Jerković-Mujkić A; University of Sarajevo-Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and Herzegovina., Žujo B; University of Sarajevo-Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and Herzegovina., Avdić M; International Burch University, Faculty of Engineering, Natural and Medical Sciences, Department of Genetics and Bioengineering, Francuske revolucije bb, 71210 Ilidža, Bosnia and Herzegovina; Academy of Sciences and Arts of Bosnia and Herzegovina, Center for Disease Control and Geohealth Studies, Bistrik 7, 71000 Sarajevo, Bosnia and Herzegovina., Hukić M; Academy of Sciences and Arts of Bosnia and Herzegovina, Center for Disease Control and Geohealth Studies, Bistrik 7, 71000 Sarajevo, Bosnia and Herzegovina; Institute for Biomedical Diagnostics and Research Nalaz, Čekaluša 69, 71000 Sarajevo, Bosnia and Herzegovina., Omeragić E; University of Sarajevo-Faculty of Pharmacy, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina., Osmanović A; University of Sarajevo-Faculty of Pharmacy, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina., Špirtović-Halilović S; University of Sarajevo-Faculty of Pharmacy, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina., Ahmetovski S; University of Sarajevo-Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and Herzegovina., Mujkanović S; University of Sarajevo-Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, 71000 Sarajevo, Bosnia and Herzegovina., Pramenković E; International Burch University, Faculty of Engineering, Natural and Medical Sciences, Department of Genetics and Bioengineering, Francuske revolucije bb, 71210 Ilidža, Bosnia and Herzegovina., Salihović M; University of Sarajevo-Faculty of Pharmacy, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2024 Sep; Vol. 177, pp. 106114. Date of Electronic Publication: 2024 Jul 05.
DOI: 10.1016/j.fitote.2024.106114
Abstrakt: Morus alba L. is a plant with a long history of dietary and medicinal uses. We hypothesized that M. alba possesses a significant biological potential. In that sense, we aimed to generate the chemical, antimicrobial, toxicological, and molecular profile of M. alba leaf and fruit extracts. Our results showed that extracts were rich in vitamin C, phenols, and flavonoids, with quercetin and pterostilbene concentrated in the leaf, while fisetin, hesperidin, resveratrol, and luteolin were detected in fruit. Extracts exhibited antimicrobial activity against all tested bacteria, including multidrug-resistant strains. The widest inhibition zones were in Staphylococcus aureus ATCC 33591. The values of the minimum inhibitory concentration ranged from 15.62 μg/ml in Enterococcus faecalis to 500 μg/ml in several bacteria. Minimum bactericidal concentration ranged from 31.25 μg/ml to 1000 μg/ml. Extracts impacted the biofilm formation in a concentration-dependent and species-specific manner. A significant difference in the frequency of nucleoplasmic bridges between the methanolic extract of fruit (0.5 μg/ml, 1 μg/ml, 2 μg/ml), as well as for the frequency of micronuclei between ethanolic extract of leaf (2 μg/ml) and the control group was observed. Molecular docking suggested that hesperidin possesses the highest binding affinity for multidrug efflux transporter AcrB and acyl-PBP2a from MRSA, as well as for the SARS-CoV-2 Mpro. This study, by complementing previous research in this field, gives new insights that could be of great value in obtaining a more comprehensive picture of the Morus alba L. bioactive potential, chemical composition, antimicrobial and toxicological features, as well as molecular profile.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2023. Published by Elsevier B.V.)
Databáze: MEDLINE
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