Transmission of classical scrapie using lymph node inoculum.

Autor: Frese AJ; Department of Biomedical Sciences, Iowa State University, College of Veterinary Medicine, Ames, IA, USA; Virus and Prion Research Unit, National Animal Disease Center, USDA, ARS, Ames, IA, USA; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN, USA. Electronic address: ajfrese@iastate.edu., Greenlee MHW; Department of Biomedical Sciences, Iowa State University, College of Veterinary Medicine, Ames, IA, USA. Electronic address: mheather@iastate.edu., Bian J; Virus and Prion Research Unit, National Animal Disease Center, USDA, ARS, Ames, IA, USA. Electronic address: jifeng.bian@usda.gov., Greenlee JJ; Virus and Prion Research Unit, National Animal Disease Center, USDA, ARS, Ames, IA, USA. Electronic address: Justin.Greenlee@usda.gov.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2024 Aug; Vol. 176, pp. 105348. Date of Electronic Publication: 2024 Jul 02.
DOI: 10.1016/j.rvsc.2024.105348
Abstrakt: Scrapie is a fatal, transmissible neurodegenerative disease that affects sheep and goats. Replication of PrP Sc in the lymphoid tissue allows for the scrapie agent to be shed into the environment. Brain and retropharyngeal lymph node (RPLN) from a sheep inoculated with the classical scrapie agent was used to compare infectivity of these tissues. Nine Cheviot sheep were used in this study, randomly assigned into two groups based on inocula. Group one (n = 4) received 1 mL of 10% brain homogenate and consisted of all VRQ/VRQ PRNP genotypes. Group two (n = 5) had three sheep receive 1 mL of a 10% RPLN homogenate (13-7), and two sheep receive 0.5 mL of a 10% RPLN homogenate (13-7) because of availability. Sheep in group two were also VRQ/VRQ genotyped. Brain and lymph tissues were tested by histopathology, immunohistochemistry, western blot, enzyme immunoassay, and conformational stability for PrP Sc accumulation. Both groups displayed clinical signs of ataxia, moribund, head tremors, circling, and lethargy prior to euthanizing at an average of 16.2 mpi (months post inoculation) (group one) or 19.56 mpi (group two). Additionally, brainstem tissue from both groups displayed the same apparent molecular mass by western blot examination. Spongiform lesion profiling and PrP Sc accumulation in brain and lymph tissues were similar in both groups. Conformational stability results displayed no significant difference in obex or RPLN tissue. Overall, these data suggest lymph nodes containing the classical scrapie agent are infectious to sheep, aiding in the understanding of sheep scrapie transmission.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests. Justin Greenlee reports financial support was provided by the USDA Agricultural Research Service. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Published by Elsevier Ltd.)
Databáze: MEDLINE
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