Dietary Kluyveromyces marxianus hydrolysate alters humoral immunity, jejunal morphology, cecal microbiota and metabolic pathways in broiler chickens raised under a high stocking density.

Autor:	Rassmidatta K; Department of Animal Science, Faculty of Agriculture at Kamphaeng Sean, Kasetsart University, Kamphang Saen Campus, Nakhon Pathom, 73140 Thailand., Theapparat Y; Center of Excellence in Functional Foods and Gastronomy, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand., Chanaksorn N; Innovad (Thailand) company limited, Bangplee, Samutprakarn, 10540 Thailand., Carcano P; Prosol S.p.A., 24040 Madone, Bergamo, Italy., Adeyemi KD; Department of Animal Production, Faculty of Agriculture, University of Ilorin, Ilorin, Kwara State, Nigeria., Ruangpanit Y; Department of Animal Science, Faculty of Agriculture at Kamphaeng Sean, Kasetsart University, Kamphang Saen Campus, Nakhon Pathom, 73140 Thailand. Electronic address: yowares.s@ku.th.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2024 Sep; Vol. 103 (9), pp. 103970. Date of Electronic Publication: 2024 Jun 12.
DOI:	10.1016/j.psj.2024.103970
Abstrakt:	This study investigated the impact of dietary supplementation with hydrolyzed yeast (Kluyveromyces marxianus) on growth performance, humoral immunity, jejunal morphology, cecal microbiota and metabolic pathways in broilers raised at 45 kg/m 2 . A total of 1,176 mixed sex 1-day-old Ross 308 broilers were distributed into 42 pens and randomly assigned to either the control group, the control + 250 g hydrolyzed yeast (HY)/ton, 250HY group, or the control + 500 g HY/ton, 500HY group for 42 d. HY did not affect growth performance. However, HY reduced (P < 0.05) mortality at 25 to 35 d. Dietary HY lowered the heterophil/lymphocyte ratio and enhanced the villus height/crypt depth ratio and Newcastle disease titer (P < 0.05). Compared with HY250 and the control, HY500 upregulated (P < 0.05) IL-10. HY enhanced the α diversity, inferring the richness and evenness of the ceca microbiota. HY500 had greater β diversity than the control (P < 0.05). Six bacterial phyla, namely, Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, Verrucomicrobia, and Cyanobacteria, were found. The relative abundance of Firmicutes was greater in the HY500 treatment group than in the HY250 and control groups. HY decreased the abundance of Actinobacteria. HY supplementation altered (P < 0.05) the abundance of 8 higher-level taxa consisting of 2 classes (Bacilli and Clostridia), 1 order (Lactobacillales), 1 family (Streptococcaceae), and five genera (Streptococcus, Lachnospiraceae_uc, Akkermansiaceae, PACO01270_g, and LLKB_g). HY500 improved (P < 0.05) the abundance of Bacilli, Clostridia, Lactobacillales, Streptococcaceae, Streptococcus, PACO01270_g, and Lachnospiraceae_uc, while HY250 enhanced (P < 0.05) the abundance of Akkermansiaceae and LLKB_g. HY improved the abundance of Lactobacillus and Akkermansia spp. Minimal set of pathway analyses revealed that compared with the control, both HY250 and HY500 regulated 20 metabolic pathways. These findings suggest that dietary K. marxianus hydrolysate, especially HY500, improved humoral immunity and jejunal morphology and beneficially altered the composition and metabolic pathways of the cecal microbiota in broilers raised at 45 kg/m 2 . Competing Interests: DISCLOSURES The authors declare no conflicts of interest. (Copyright © 2024. Published by Elsevier Inc.)
Databáze:	MEDLINE
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