Embedding Bioprinting of Low Viscous, Photopolymerizable Blood-Based Bioinks in a Crystal Self-Healing Transparent Supporting Bath.

Autor: Caiado Decarli M; MERLN Institute for Technology-Inspired Regenerative Medicine, Department of Complex Tissue Regeneration, Maastricht University, Universiteitssingel 40, Maastricht, 6229 ER, The Netherlands.; Department of Biomaterials & Biomedical Technology, University Medical Center Groningen/University of Groningen, A. Deusinglaan 1, Groningen, AV 9713, The Netherlands., Ferreira HP; i3S - Instituto de Investigação e Inovação em Saúde/INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen 208, Porto, 4200-180, Portugal.; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, 4050-313, Portugal., Sobreiro-Almeida R; CICECO - Department of Chemistry, Aveiro Institute of Materials, University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal., Teixeira FC; MERLN Institute for Technology-Inspired Regenerative Medicine, Department of Complex Tissue Regeneration, Maastricht University, Universiteitssingel 40, Maastricht, 6229 ER, The Netherlands., Correia TR; CICECO - Department of Chemistry, Aveiro Institute of Materials, University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal., Babilotte J; MERLN Institute for Technology-Inspired Regenerative Medicine, Department of Complex Tissue Regeneration, Maastricht University, Universiteitssingel 40, Maastricht, 6229 ER, The Netherlands., Olijve J; Rousselot Biomedical, Expertise Center, Meulestedekaai 81, Ghent, 9000, Belgium., Custódio CA; CICECO - Department of Chemistry, Aveiro Institute of Materials, University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal.; Metatissue, PCI, Creative Science Park Aveiro Region, Via do Conhecimento, Ílhavo, 3830-352, Portugal., Gonçalves IC; i3S - Instituto de Investigação e Inovação em Saúde/INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen 208, Porto, 4200-180, Portugal.; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, 4050-313, Portugal., Mota C; MERLN Institute for Technology-Inspired Regenerative Medicine, Department of Complex Tissue Regeneration, Maastricht University, Universiteitssingel 40, Maastricht, 6229 ER, The Netherlands., Mano JF; CICECO - Department of Chemistry, Aveiro Institute of Materials, University of Aveiro, Campus Universitário de Santiago, Aveiro, 3810-193, Portugal., Moroni L; MERLN Institute for Technology-Inspired Regenerative Medicine, Department of Complex Tissue Regeneration, Maastricht University, Universiteitssingel 40, Maastricht, 6229 ER, The Netherlands.
Jazyk: angličtina
Zdroj: Small methods [Small Methods] 2024 Jul 06, pp. e2400857. Date of Electronic Publication: 2024 Jul 06.
DOI: 10.1002/smtd.202400857
Abstrakt: Protein-based hydrogels have great potential to be used as bioinks for biofabrication-driven tissue regeneration strategies due to their innate bioactivity. Nevertheless, their use as bioinks in conventional 3D bioprinting is impaired due to their intrinsic low viscosity. Using embedding bioprinting, a liquid bioink is printed within a support that physically holds the patterned filament. Inspired by the recognized microencapsulation technique complex coacervation, crystal self-healing embedding bioprinting (CLADDING) is introduced based on a highly transparent crystal supporting bath. The suitability of distinct classes of gelatins is evaluated (i.e., molecular weight distribution, isoelectric point, and ionic content), as well as the formation of gelatin-gum arabic microparticles as a function of pH, temperature, solvent, and mass ratios. Characterizing and controlling this parametric window resulted in high yields of support bath with ideal self-healing properties for interaction with protein-based bioinks. This support bath achieved transparency, which boosted light permeation within the bath. Bioprinted constructs fully composed of platelet lysates encapsulating a co-culture of human mesenchymal stromal cells and endothelial cells are obtained, demonstrating a high-dense cellular network with excellent cell viability and stability over a month. CLADDING broadens the spectrum of photocrosslinkable materials with extremely low viscosity that can now be bioprinted with sensitive cells without any additional support.
(© 2024 The Author(s). Small Methods published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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